4-methylumbelliferyl

4-Methylumbelliferyl phosphate (4-MUP) 是碱性磷酸酶的荧光底物。

4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt) (4-MU-Gal-6S) is a fluorogenic substrate used to quantify N-acetylgalactosamine-6-sulphatase (GALNS) activity. 4-MU-Gal-6S is cleaved by GALNS to release the fluorescent moiety 4-MU. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases. It has been used to detect Morquio disease type A, a lysosomal storage disorder in which GALNS is deficient. 4-MU-Gal-6S can be used to assess GALNS activity in a very small blood volume to determine the extent of deficiency.

4-Methylumbelliferyl 2-sulfamino-2-deoxy-α-D-Glucopyranoside (4-MU-α-GlcNS) is a fluorogenic substrate of heparin sulphamidase. Heparin sulphamidase cleaves 4-MU-α-GlcNS to yield 4-MU-α-GlcNH2, which is then cleaved by α-glucosaminidase to release the fluorescent product 4-MU, which displays an emission maxima of 445-454 nm. The excitation maxima for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. 4-MU-α-GlcNS has been used to quantify heparin sulphamidase deficiencies associated with Mucopolisaccaridosis IIIA and other lysosomal disorders.

4-Methylumbelliferyl phosphate disodium (4-MUP) 是一种阴离子有机磷酸酯，作为酸性和碱性磷酸酶（phosphatase）的荧光底物，同时也用作神经毒剂模拟剂。

4-Methylumbelliferyl β-cellotrioside为β-糖苷酶特异性显色底物，属于纤维素类荧光探针，应用于相关酶活性检测。

4-Methylumbelliferyl palmitate 是用于测量人白细胞中酸性脂肪酶的优良荧光探针。该化合物的荧光特性受pH值和溶剂类型的显著影响。在近中性水溶液中，4-Methylumbelliferyl palmitate 以中性形态存在，并在445 nm发射较强荧光；而在微弱碱性环境中，则以阴离子形态存在，同样在445 nm处展现较强的荧光。

4-Methylumbelliferyl butyrate (4-MUB) 是一种香豆素衍生的荧光底物，专用于检测 M. catarrhalis 中的 C4-酯酶活性。该化合物在反应中转化生成发蓝光的 4-甲基伞形酮 (4-MU)。

4-Methylumbelliferyl sulfate (potassium) 是一种荧光底物，常用于生化和生物医学研究中检测硫酸酯酶活性。它由连接到荧光分子上的硫酸基组成，可以被硫酸酯酶裂解，释放出高荧光产物，通过荧光显微镜或光谱法检测。使用4-Methylumbelliferyl sulfate作为硫酸酯酶底物，可以准确检测和定量各种生物样品中的这些酶。

N-(4-Methylumbelliferyl)-maleinimid是一种多功能染料。染料在生物实验中是关键工具，用于观察和分析细胞结构、追踪生物分子、评估细胞功能和类型、检测生物分子、研究组织病理以及监测微生物，其应用范围广泛，涵盖基础科学研究至临床诊断。此外，染料在纺织品染色、功能性纺织品处理、食品色素和染料敏化太阳能电池等领域也有广泛应用。

4-Methylumbelliferyl β-D-cellobioside 是 Cellulase 的一种底物。它可以通过水解生成具有荧光特性的 4-甲基伞形酮 (4-methylumbelliferone)，用于研究纤维素酶的动力学特性。

4-Methylumbelliferyl heptanoate (4-Methylumbelliferyl enanthate) 是一种脂肪酶荧光底物，可检测细胞介导的细胞毒性和细胞增殖。

4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG) 是一种荧光底物 (λex=362 nm，λem=445 nm)。

4-Methylumbelliferyl-α-L-iduronide 2-sulfate (4-MU-α-idoa 2-sulfate) sodium 是艾杜糖苷酸-2-硫酸酯酶 (I2S) 的一种荧光底物。此化合物可被溶酶体酶 α-L-艾杜糖苷酶 (IDUA) 水解，从而释放荧光团4-Methylumbelliferone，并用于检测 Hurler 综合征。

4-Methylumbelliferyl-β-D-galactoside (MUG) is a fluorogenic substrate for β-galactosidase (β-gal).1Hydrolysis of MUG by β-gal releases the fluorescent moiety 4-MU, which displays a pH-dependent excitation maximum of 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively, and an emission maximum between 445-454 nm.2MUG has been used to detect β-gal activity in intact bacteria, yeast, and mammalian cells.1 1.Vidal-Aroca, F., Giannattasio, M., Brunelli, E., et al.One-step high-throughput assay for quantitative detection of β-galactosidase activity in intact Gram-negative bacteria, yeast, and mammalian cellsBiotechniques40(4)433-434(2006) 2.Zhi, H., Wang, J., Wang, S., et al.Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsuleJ. Spectrosc.1(1)147128(2013)

4-Methylumbelliferyl oleate 是脂肪酶的荧光底物(Ex/Em=320/450 nm),可被脂肪酶裂解，可测定胰腺抑制活性。

4-Methylumbelliferyl-α-L-fucopyranoside是α-L-fucosidase的荧光底物。当α-L-fucosidase酶解此底物后，会释放出4-methylumbelliferone (4-ΜU)，其荧光可用于定量α-L-fucosidase活性。4-ΜU的激发/发射峰值分别为350/440 nm。

4-Methylumbelliferyl-N-acetyl-α-D-glucosaminide 作为一种荧光底物，主要应用于 α-N-acetylglucosaminidase 的检测。

4-MUNANA(4-Methylumbelliferyl-N-acetyl-α-D-Neuraminic Acid sodium salt) 是用于神经氨酸酶 (neuraminidase) 活性测定的荧光底物，是神经氨酸酶底物类似物。