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Ephrin B1/EFNB1 Protein, Mouse, Recombinant (His)

产品编号 TMPY-01753

别名: ephrin-B1, EFL-3, Eplg2, Epl2, Elk-L, Cek5-L, Stra1, Lerk2, LERK-2

Ephrin B1/EFNB1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.3 kDa and the accession number is P52795.

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Ephrin B1/EFNB1 Protein, Mouse, Recombinant (His)

规格	价格/CNY		货期	数量
200 μg	￥ 4,420		5日内发货

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参考文献

生物活性	Measured by its ability to compete with mouse EphrinB1 / Fc for binding to immobilized mouse EphB3 in a functional ELISA assay.
产品描述	Ephrin B1/EFNB1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.3 kDa and the accession number is P52795.

种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P52795
别名	ephrin-B1, EFL-3, Eplg2, Epl2, Elk-L, Cek5-L, Stra1, Lerk2, LERK-2
蛋白构建	The Mouse EFNB1 (NP_034240.1) extracellular domain (Met 1-Ser 229) was expressed, fused with a polyhistidine tag at the C-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	23.3 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

参考文献

1. Davy A, et al. (2004) Ephrin-B1 forward and reverse signaling are required during mouse development. Genes Dev. 18(5): 572-83. 2. Compagni A, et al. (2003) Control of skeletal patterning by ephrinB1-EphB interactions. Dev Cell. 5(2): 217-30. 3. Wieland I, et al. (2004) Mutations of the ephrin-B1 gene cause craniofrontonasal syndrome. Am J Hum Genet. 74(6): 1209-15.

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Keywords

Ephrin B1/EFNB1 Protein, Mouse, Recombinant (His) EFL3 ephrin-B1 EFL-3 Eplg2 EFL 3 Epl2 Elk-L Cek5-L Stra1 LERK 2 Lerk2 LERK-2 recombinant recombinant-proteins proteins protein

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