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Ephrin B1/EFNB1 Protein, Human, Recombinant (His)

Ephrin B1/EFNB1 Protein, Human, Recombinant (His)

产品编号 TMPY-01171
别名: EFL3, CFND, EPLG2, CFNS, EFB1, Elk-L, LERK2, ephrin-B1

Ephrin B1/EFNB1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 24.5 kDa and the accession number is P98172.

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Ephrin B1/EFNB1 Protein, Human, Recombinant (His)
规格 价格/CNY 货期 数量
100 μg ¥ 1,950 5日内发货
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产品目录号及名称: Ephrin B1/EFNB1 Protein, Human, Recombinant (His) (TMPY-01171)
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生物活性
技术参数
产品性质
参考文献
生物活性 1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant human EphrinB1 at 10 μg/ml (100 μl/well) can bind human EphB6 with a linear range of 0.16-4 μg/ml.
产品描述 Ephrin B1/EFNB1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 24.5 kDa and the accession number is P98172.
种属 Human
表达系统 HEK293 Cells
标签 C-His
蛋白编号 P98172
别名 EFL3, CFND, EPLG2, CFNS, EFB1, Elk-L, LERK2, ephrin-B1
蛋白构建 The Human EFNB1 (NP_004420.1) extracellular domain (Met 1-Lys 237) was fused with a polyhistidine tag at the C-terminus.
蛋白纯度 > 95 % as determined by SDS-PAGE
分子量 24.5 kDa (predicted)
内毒素 < 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice.

研究背景 Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

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Keywords

Ephrin B1/EFNB1 Protein, Human, Recombinant (His) LERK 2 EPLG-2 EFL3 CFND EFL-3 EPLG2 CFNS EFB1 Elk-L LERK-2 EFB 1 EPLG 2 LERK2 EFB-1 ephrin-B1 EFL 3 recombinant recombinant-proteins proteins protein

 

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