Ephrin A5/EFNA5 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48 kDa and the accession number is P97605.
生物活性 | Measured by its binding ability in a functional ELISA. Immobilized recombinant Rat EPHA4 Protein (His Tag) at 10 µg/mL (100 μl/well) can bind Recombinant rat EFNA5-Fc with a linear range of 7.8-12.5 ng/ml. |
产品描述 | Ephrin A5/EFNA5 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48 kDa and the accession number is P97605. |
种属 | Rat |
表达系统 | HEK293 Cells |
标签 | C-hFc |
蛋白编号 | P97605 |
别名 | ephrin-A5 |
蛋白构建 | The mature form of Rat EFNA5 (P97605) (Met 1-Glu 202) was fused with the Fc region of Human IgG1 at the C-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 48 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion. |
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Ephrin A5/EFNA5 Protein, Rat, Recombinant (hFc) ephrin-A5 recombinant recombinant-proteins proteins protein