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Ephrin A1/EFNA1 Protein, Human, Recombinant (His)

产品编号 TMPY-01210

Ephrin A1/EFNA1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.8 kDa and the accession number is P20827-1.

Ephrin A1/EFNA1 Protein, Human, Recombinant (His)

产品编号 TMPY-01210

Ephrin A1/EFNA1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.8 kDa and the accession number is P20827-1.

规格	价格	库存	数量
100 μg	¥ 2,940	5日内发货

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产品信息

生物活性	Measured by its binding ability in a functional ELISA. Immobilized Human Ephrin-A1 His at 2 μg/ml (100 μl/well) can bind Human EphA1 hFc, the EC50 of Human EphA1 hFc is 8.0-48.0 ng/mL.
产品描述	Ephrin A1/EFNA1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.8 kDa and the accession number is P20827-1.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P20827-1
别名	TNFAIP4,LERK-1,LERK1,EPLG1,ephrin-A1,EFNA1,EFL1,ECKLG,B61
蛋白构建	A DNA sequence encoding the human Ephrin-A1 (NP_004419.2) without the propeptide (Met 1-Ser182) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Asp 19
蛋白纯度	> 97 % as determined by SDS-PAGE
分子量	20.8 kDa (predicted); 26 kDa (reducing condition, due to glycosylation)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	EPH-related receptor tyrosine kinase ligand 1 (abbreviated as Ephrin-A1) also known as ligand of eph-related kinase 1 or EFNA1, is a member of the ephrin (EPH) family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A1/EFNA1 and its Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A1 and one of its receptor EphA2 were expressed in xenograft endothelial cells and also tumor cells and play a role in human cancers, at least in part by influencing tumor neovascularization.