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EFNB2A Protein, Danio rerio (zebrafish), Recombinant (hFc)

产品编号 TMPY-03632

别名: EFNB2A, efnb2, id:ibd572

EFNB2A Protein, Danio rerio (zebrafish), Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48.9 kDa and the accession number is O73874.

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EFNB2A Protein, Danio rerio (zebrafish), Recombinant (hFc)

规格	价格/CNY		货期	数量
100 μg	￥ 2,940		5日内发货

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参考文献

生物活性	Measured by its binding ability in a functional ELISA. Immobilized human EphB4-His at 10 μg/ml (100 μl/well) can bind zebrafish EFNB2A-Fc, The EC50 of zebrafish EFNB2A-Fc is 12.8-30.1 ng/ml.
产品描述	EFNB2A Protein, Danio rerio (zebrafish), Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48.9 kDa and the accession number is O73874.

种属	Danio rerio (zebrafish)
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	O73874
别名	EFNB2A, efnb2, id:ibd572
蛋白构建	The zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed with the Fc region of Human IgG1 at the C-terminus.
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	48.9 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, PH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Efnb2a, also known as ephrin B2a, belongs to the ephrin family. Members of this family are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Efnb2a binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Efnb2a, together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration.

参考文献

1. Shaw KM. et al., 2006, Dev Dyn. 235 (7): 1753-60. 2. Kemp HA. et al., 2009, Dev Biol. 327 (2): 313-26.Patten SA. et al., 2012, PLoS One. 7 (2): e31650.

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Keywords

EFNB2A Protein, Danio rerio (zebrafish), Recombinant (hFc) EFNB2A efnb2 id:ibd572 recombinant recombinant-proteins proteins protein

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