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CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPY-01985
CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 24 kDa and the accession number is P31994-2.
规格价格库存数量
20 μg¥ 2,930现货
100 μg¥ 6,8305日内发货
200 μg¥ 11,6505日内发货
500 μg¥ 23,5405日内发货
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产品信息

生物活性
Loaded Biotinylated Human FcγRIIB/C / CD32b/c protein (His & Avi Tag) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.2 μM as determined in a BLI assay (QC tested).
CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi), Biotinylated
产品描述
CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 24 kDa and the accession number is P31994-2.
种属
Human
表达系统
HEK293 Cells
标签His, Avi
蛋白编号P31994-2
别名
IGFR2,FCGR2A,FCGR21,FCGR2,FcGR,FCG2,Fc γ RIIB,Fc γ RII,Fc gamma RIIB,Fc gamma RII,Fc fragment of IgG, low affinity IIb, receptor (CD32),CDw32,CD32B,CD32A,CD32
蛋白构建
A DNA sequence encoding the extracellular domain (Ala 46-Ile 224) of mature human CD32b (NP_001002274.1) protein was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ala 46
蛋白纯度
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi), BiotinylatedCD32B/Fcgr2b Protein, Human, Recombinant (His & Avi), Biotinylated
分子量24 kDa (predicted); 35 kDa (reducing condition, due to glycosylation)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.

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