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Anti-PTGS1 Antibody (7Y100)
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产品编号 TMAH-01014
别名 PTGS 1, Prostaglandin-endoperoxide synthase 1, Prostaglandin H2 synthase 1, Prostaglandin G/H synthase 1, PHS 1, PGHS-1, PGH synthase 1, EC 1.14.99.1, Cyclooxygenase-1, COX-1, COX1
Anti-PTGS1 Antibody (7Y100) 是一种抗体，靶向 PTGS1。Anti-PTGS1 Antibody (7Y100) 可用于 ELISA, WB, FCM。
Anti-PTGS1 Antibody (7Y100)
Anti-PTGS1 Antibody (7Y100)
还可以产品编号 TMAH-01014 别名 PTGS 1, Prostaglandin-endoperoxide synthase 1, Prostaglandin H2 synthase 1, Prostaglandin G/H synthase 1, PHS 1, PGHS-1, PGH synthase 1, EC 1.14.99.1, Cyclooxygenase-1, COX-1, COX1
Anti-PTGS1 Antibody (7Y100) 是一种抗体，靶向 PTGS1。Anti-PTGS1 Antibody (7Y100) 可用于 ELISA, WB, FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,195 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-PTGS1 Antibody (7Y100) is an antibody targeting PTGS1. Anti-PTGS1 Antibody (7Y100) can be used in ELISA, WB, FCM. |
| 别名 | PTGS 1, Prostaglandin-endoperoxide synthase 1, Prostaglandin H2 synthase 1, Prostaglandin G/H synthase 1, PHS 1, PGHS-1, PGH synthase 1, EC 1.14.99.1, Cyclooxygenase-1, COX-1, COX1 |
| Ig Type | Rabbit IgG |
| 克隆号 | 7Y100 |
| 交叉反应 | Human, Mouse |
| 验证活性 | 1. Western Blot -Positive WB detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissue -All lanes: PTGS1 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 69, 65, 62, 57, 72, 73 kDa -Observed band size: 72 kDa 2. Overlay histogram showing Hela cells stained with TMAH-01014 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | |
| 推荐剂量 | WB:1:500-1:5000; FCM:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human COX1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cancer, Cardiovascular, Immunology, Metabolism, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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