Anti-OGT Antibody (7A669)

Name: Anti-OGT Antibody (7A669)
Brand: TargetMol
SKU: TMAH-00844
Rating: 4.5 (1 reviews)

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产品编号 TMAH-00844

Anti-OGT Antibody (7A669) 是一种抗体，靶向 OGT。Anti-OGT Antibody (7A669) 可用于 ELISA, WB, IHC。

Anti-OGT Antibody (7A669)

还可以

产品编号 TMAH-00844

Anti-OGT Antibody (7A669) 是一种抗体，靶向 OGT。Anti-OGT Antibody (7A669) 可用于 ELISA, WB, IHC。

规格	价格	库存	数量
50 μL	¥ 1,305	5日内发货
100 μL	¥ 2,180	5日内发货

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生物活性

产品描述	Anti-OGT Antibody (7A669) is an antibody targeting OGT. Anti-OGT Antibody (7A669) can be used in ELISA, WB, IHC.
Ig Type	Rabbit IgG
克隆号	7A669
交叉反应	Human, Mouse, Rat
验证活性	1. Western Blot -Positive WB detected in: NIH/3T3 whole cell lysate, HL-60 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate -All lanes: OGT antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 117, 104, 116, 75 kDa -Observed band size: 117 kDa 2. IHC image of TMAH-00844 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
应用	ELISA, WB, IHC
推荐剂量	WB:1:500-1:5000; IHC:1:50-1:200.
抗体种类	Monoclonal
亚细胞定位	Nucleus. Cytoplasm.; [Isoform 2]: Mitochondrion. Membrane.; [Isoform 3]: Cytoplasm. Nucleus. Cell membrane. Mitochondrion membrane. Cell projection.; [Isoform 4]: Cytoplasm. Nucleus.
构建方式	Recombinant Antibody
纯化方式	Affinity-chromatography
性状	Liquid
缓冲液	Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景	Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Probably by glycosylating KMT2E/MLL5, stabilizes KMT2E/MLL5 by preventing its ubiquitination. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues. O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex. Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2. O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity. Regulates mitochondrial motility in neurons by mediating glycosylation of TRAK1. Glycosylates HOXA1. O-glycosylates FNIP1. the mitochondrial isoform (mOGT) is cytotoxic and triggers apoptosis in several cell types including INS1, an insulinoma cell line.

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	A synthetic peptide: Human OGT / O-Linked N-Acetylglucosamine Transferase
抗原种属	Human
基因ID	8473
Uniprot ID	O15294
研究领域	Neuroscience, Signal transduction