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顶部生物活性储存和溶解信息计算器

Anti-NRAS Antibody (5T588)

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Anti-NRAS Antibody (5T588)
产品编号 TMAC-03538
别名 NS6, NRAS1, N-ras, neuroblastoma RAS viral (v-ras) oncogene homolog, NCMS, CMNS, ALPS4

Anti-NRAS Antibody (5T588) 是一种 Rabbit 抗体，靶向 NRAS。Anti-NRAS Antibody (5T588) 可用于 WB,ICC/IF,IP。

Anti-NRAS Antibody (5T588)

Anti-NRAS Antibody (5T588)

Rating icon 还可以
Anti-NRAS Antibody (5T588)
产品编号 TMAC-03538 别名 NS6, NRAS1, N-ras, neuroblastoma RAS viral (v-ras) oncogene homolog, NCMS, CMNS, ALPS4

Anti-NRAS Antibody (5T588) 是一种 Rabbit 抗体，靶向 NRAS。Anti-NRAS Antibody (5T588) 可用于 WB,ICC/IF,IP。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-NRAS Antibody (5T588) is a Rabbit antibody targeting NRAS. Anti-NRAS Antibody (5T588) can be used in WB,ICC/IF,IP.
别名NS6, NRAS1, N-ras, neuroblastoma RAS viral (v-ras) oncogene homolog, NCMS, CMNS, ALPS4
Ig Type
IgG
交叉反应
Human,Mouse,Rat,zebrafish
验证活性
1. Western blot analysis of Ras on different lysates using anti-Ras antibody at 1/1,000 dilution. Positive control: Lane 1: 293T, Lane 2: MCF-7, Lane 3: Hela, Lane 4: zebrafish.
2. ICC staining Ras in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Ras in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Ras in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
WB
ICC/IF
IP
推荐剂量
WB: 1:1000-2000; ICC/IF: 1:100-500
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode guanine nucleotide-binding proteins that are ubiquitously expressed in vertebrate cells. c-H- and c-K-Ras are cellular homologs of the v-H and v-K-Ras sequences originally isolated from the Harvey and Kirsten strains of rat sarcoma virus. Ras-encoded proteins bind GDP and GTP with high affinity and possess a low level intrinsic GTPase activity that can be stimulated over 100-fold by interaction with cytosolic GTPase activating protein (GAP), a potential effector for Ras p21 function. Point mutations at amino acids 12, 13, 59 and 61 within domains responsible for GTP binding and hydrolysis activate Ras proteins to their oncogenic form and block the ability of the GTPase activity to be stimulated by GAP. Several additional proteins with GAP activity have been identified and shown to interact with p21 Ras or other members of the Ras gene family.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P01111
化学信息
分子量Theoretical: 21 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

NS-6NS 6NRAS-1NRAS 1ALPS-4ALPS 4

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