Anti-LAG3 Antibody (5D620) is a Mouse antibody targeting LAG3. Anti-LAG3 Antibody (5D620) can be used in ELISA, WB, IHC, FC.

IgG1

5D620

Human

1. Western Blot
-Positive WB detected in: 1 lane: Recombinant proteins with LAG3; 2 lane: 293F whole cell lysate transfected with LAG3
-All lanes: LAG3 antibody at 1:2000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 76, 88 KDa
-Observed band size: 76, 88 KDa
-Exposure time:5min
2. IHC image of TMAH-00681 diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00681 diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
4. Overlay Peak curve showing A549 cells stained with TMAH-00681 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC, FC

Monoclonal

Unconjugated

Recombinant Protein: Human Lymphocyte activation gene 3 Protein (29-450AA)

Human

3902

Immunology