Anti-HSPA8 Antibody (3I632) is a Mouse antibody targeting HSPA8. Anti-HSPA8 Antibody (3I632) can be used in ELISA, WB, IHC, IF, FC.

IgG1

3I632

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
-All lanes HSPA8 antibody at 1:2000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 70~75 KDa
-Observed band size: 70~75 KDa
-Exposure time: 5min
2. Western Blot
-Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg
-All lanes: HSPA8 antibody at 1:2000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 70~75 KDa
-Observed band size: 70~75 KDa
-Exposure time: 5min
3. Western Blot
-Positive WB detected in: 20μg Hela whole cell lysate
HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 70~75 KDa
-Observed band size: 70~75 KDa
-Exposure time: 5min
4. IHC image of TMAH-00578 diluted at 1:220 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
5. IHC image of TMAH-00578 diluted at 1:220 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
6. Immunofluorescence staining of Hela cells with TMAH-00578 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
7. Immunofluorescence staining of MCF-7 cells with TMAH-00578 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
8. Immunofluorescence staining of PC-3 cells with TMAH-00578 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
9. Overlay histogram showing MCF-7 cells stained with TMAH-00578 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the primary antibody at 1:200 for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC, IF, FC

Monoclonal

Unconjugated

Recombinant Protein: Human Heat shock cognate 71 kDa Protein (2-646AA)

Human

3312

Immunology