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Anti-Aquaporin-9/AQP9 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Aquaporin-9/AQP9。Anti-Aquaporin-9/AQP9 Polyclonal Antibody 可用于 FCM。
Anti-Aquaporin-9/AQP9 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Aquaporin-9/AQP9。Anti-Aquaporin-9/AQP9 Polyclonal Antibody 可用于 FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-Aquaporin-9/AQP9 Polyclonal Antibody is a Rabbit antibody targeting Aquaporin-9/AQP9. Anti-Aquaporin-9/AQP9 Polyclonal Antibody can be used in FCM. |
Ig Type | IgG |
交叉反应 | Human (predicted:Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig) |
验证活性 | Blank control: A549 (blue). Primary Antibody: Rabbit Anti-AQP9/AF647 Conjugated antibody (TMAB-00143/AF647), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647 (orange),used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min). The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (TMAB-00143/AF647, 1 μg/1x10^6 cells) for 30 min on ice. |
应用 | |
推荐剂量 | FCM: 1μg /test |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Membrane; Multi-pass membrane protein. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. A new member of the AQP family, AQP9, has been cloned from human leukocytes. It is homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295aa) is primarily expressed in peripheral leukocytes, and in lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it does not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. |
免疫原 | KLH conjugated synthetic peptide: human AQP9 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Cancer,Channels |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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