fluorescent probes

Benzofuroxan 被用作合成其他化合物的起始材料，如药物和其他化学品；也用于聚合物的合成；还被用于合成荧光染料，并作为检测某些分子的荧光探针。

5-Aminofluorescein hydrochloride (5-AF hydrochloride)作为一种高效的荧光标记试剂，在细胞成像和分子探针领域得到了广泛应用。该化合物能够实现蛋白质位置的精确观测及其动态变化的追踪，为生物学研究提供关键视角。此外，5-Aminofluorescein hydrochloride在探测生物分子相互作用方面也显示出其重要价值，促进对复杂生物机制的理解。

CDy9 is a highly selective fluorescent probe for the detection and isolation of mESC using fluorescence microscopy and flow cytometry. CDy9 allows the detection and isolation of intact stem cells with marginal impact on their function and capabilities. CD

DCP-Rho1 is a fluorescent probe for the detection of sulfenic acid-containing proteins.1,2 It displays excitation emission maxima of 560 581 nm, respectively, and has been used to visualize protein oxidation sites in situ. |1. Klomsiri, C., Rogers, L.C., Soito, L., et al. Endosomal H2O2 production leads to localized cysteine sulfenic acid formation on proteins during lysophosphatidic acid-mediated cell signaling. Free Rad. Biol. Med. 71, 49-60 (2014).|2. Holmila, R.J., Vance, S.A., Chen, X., et al. Mitochondria-targeted probes for imaging protein sulfenylation. Sci. Rep. 8(1), 6635 (2018).

ICAAc is a solvatochromic fluorescent pH probe.1 As the polarity of the solvent increases, the emission wavelength of ICAAc increases. It displays excitation/emission maxima of 466/553, 431/515, and 418/503 nm in water, dioxane, and hexane, respectively. The absorption maximum of ICAAc decreases with increasing pH. It displays absorption/emission maxima of 470/554 and 428/553 nm at pH 3 and 11, respectively, in aqueous Britton-Robinson buffer, and the fluorescence intensity increases as pH decreases. ICAAc can be used for live cell applications. |1. Nagy, M., Racz, D., Nagy, Z.L., et al. Amino-isocyanoacridines: Novel, tunable solvatochromic fluorophores as phystiological pH probes. Sci. Rep. 9, 8250 (2019).

1,3,4,6-Tetra-O-acetyl-2-azido-2-deoxy-α-D-mannopyranose is an analog of N-acetylmannosamine (ManNAc) and a building block.1,2It has been used as a precursor in the synthesis of differently substituted trimers of the group AN. meningitidiscapsular polysaccharide repeating unit for use in immunological experiments.2It has also been used in the synthesis of fluorescent probes for lysosomal labeling.3

monoMICAAc is a solvatochromic fluorescent pH probe.1 As the polarity of the solvent increases, the emission wavelength of monoMICAAc increases. It displays excitation emission maxima of 425 491, 437 515, and 472 554 nm in hexane, dioxane, and water, respectively. The absorption maximum of monoMICAAc decreases with increasing pH. It displays absorbance emission maxima of 475 553 and 446 553 nm at pH 3 and 11, respectively, in aqueous Britton-Robinson buffer, and the fluorescence intensity increases as pH decreases. monoMICAAc can be used for live cell fluorescent applications. |1. Nagy, M., Racz, D., Nagy, Z.L., et al. Amino-isocyanoacridines: Novel, tunable solvatochromic fluorophores as phystiological pH probes. Sci. Rep. 9, 8250 (2019).

Quin-2 is a high-affinity fluorescent calcium indicator (Kd = 115 nM for calcium). It displays high selectivity for calcium, as it is not affected by sodium gradients, membrane potential, or intracellular pH. High affinity probes like quin-2 are ideal for monitoring low levels of calcium, as are found in resting cells. Loadings of up to 2 mM quin-2 are without serious toxic effects, so quin-2 may be used to buffer intracellular calcium transients. Excitation emission maxima for quin-2 are 339 and 492 nm, respectively.

8Br-HA is an inhibitor of fragile histidine triad diadenosine triphosphatase (FHIT; IC50= 0.12 μM).1It inhibits the growth of HCC827 and H460 lung cancer cells (GI50s = 0.87 and 5.9 μM, respectively). 1.Kawaguchi, M., Sekimoto, E., Ohta, Y., et al.Synthesis of fluorescent probes targeting tumor-suppressor protein FHIT and Identification of apoptosis-inducing FHIT inhibitorsJ. Med. Chem.64(13)9567-9576(2021)

N-ε-propargyloxycarbonyl-L-lysine (H-L-Lys(Poc)-OH) is an unnatural amino acid (UAA) derived from lysine. It is commonly employed for bio-conjugation purposes, specifically for attaching fluorescent probes in a variety of organisms ranging from E. coli to mammalian cells, including animals.

DMT-5Me-dC(Bz)-CE Phosphoramidite 用于生产锁定核酸（LNAs），旨在优化荧光寡核苷酸探针的设计，从而提升其光谱性质和对目标的结合能力。

cRGDfK-thioacetyl ester 为具选择性亲和力于整合素（integrins）之生物活性多肽。可应用于修饰NIR荧光探针，实现癌症靶向成像。

9H-Fluoren-9-amine hydrochloride是一种芴衍生物，在染料、荧光探针和药物合成中用作重要中间体。

Cy5-UTP sodium (10mM in Water) 是一种 Cyanine5 标记的 UTP 类似物，其用于体外转录反应生成荧光探针，激发 发射波长为 650 665 nm。适用于 FISH 和多色荧光分析，尤其是与 Cy5-UTP 联合使用的双色表达阵列。