Name: TLR4 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02904
Rating: 4.5 (1 reviews)

规格

价格

库存

数量

5 μg

¥ 429

3日内发货

10 μg

¥ 697

3日内发货

20 μg

¥ 1,150

3日内发货

50 μg

¥ 2,260

3日内发货

100 μg

¥ 3,820

现货

200 μg

¥ 6,680

5日内发货

500 μg

¥ 13,900

5日内发货

1 mg

¥ 24,900

5日内发货

生物活性

产品描述	TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has also been designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independent inflammatory responses triggered by Ni(2+).
生物活性	1.Immobilized TLR4 Protein, Human, Recombinant (His) at 1 μg/mL (100 μL/well) on His Tag Antibody, Mouse MAb precoated (5 μg/mL, 100 μL/well) can bind Anti-TLR4 Antibody, the EC50 is 4-15 ng/mL (QC tested). 2.Immobilized Anti-TLR4 Antibody at 2 μg/mL (100 μL/well) can bind TLR4 Protein, Human, Recombinant (His), the EC50 is 4-15 ng/mL (Routinely tested).
研究背景	TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has also been designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independent inflammatory responses triggered by Ni(2+).
种属	Human
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	O00206-1
蛋白构建	A DNA sequence encoding the human TLR4 (Met1-Lys631) (O00206-1) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Glu 24
蛋白纯度	≥ 87 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白性状	Lyophilized powder or solution
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 500 mM NaCl, pH 6. 0, 10% glycerol or Supplied as sterile 20 mM PB, 500 mM NaCl, 10% Glycerol, pH 6. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
别名	toll-like receptor 4, TOLL, TLR-4, TLR4, CD284, ARMD10
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.