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LIGHT/TNFSF14 Protein, Human, Recombinant (Avi & Fc), Biotinylated

产品编号 TMPY-05643

LIGHT/TNFSF14 Protein, Human, Recombinant (Avi & Fc), Biotinylated is expressed in HEK293 mammalian cells with AVI and Fc tag. The predicted molecular weight is 46.7 kDa and the accession number is O43557-1.

LIGHT/TNFSF14 Protein, Human, Recombinant (Avi & Fc), Biotinylated

产品编号 TMPY-05643

规格	价格	库存
5 μg	¥ 1,180	6-8日内发货
10 μg	¥ 1,960	6-8日内发货
20 μg	¥ 3,380	6-8日内发货
50 μg	¥ 5,460	6-8日内发货
100 μg	¥ 7,880	6-8日内发货

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产品信息

生物活性	Immobilized Human HVEM His at 2 μg/mL (100 μL/well) can bind Human LIGHT (ECD, hFc & AVI Tag), Biotinylated , the EC50 is 20-180 ng/mL.
产品描述	LIGHT/TNFSF14 Protein, Human, Recombinant (Avi & Fc), Biotinylated is expressed in HEK293 mammalian cells with AVI and Fc tag. The predicted molecular weight is 46.7 kDa and the accession number is O43557-1.
种属	Human
表达系统	HEK293 Cells
标签	N-Avi-hFc
蛋白编号	O43557-1
别名	tumor necrosis factor (ligand) superfamily, member 14,TR2,LTg,LIGHT,HVEML,CD258
蛋白构建	A DNA sequence encoding the human TNFSF14 (NP_001363816.1) (Asp74-Val240) was expressed with a N-terminal AVI tag followed by a Fc region of human IgG1 tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Gly
蛋白纯度	> 90 % as determined by SDS-PAGE.
分子量	46.7 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	LIGHT, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14 / LIGHT / CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin β receptor (LTβR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14 / LIGHT / CD258 also binds to a soluble non-signaling receptor, decoy receptor 3 (DcR3), which can modulate the function of LIGHT in vivo. TNFSF14 / LIGHT / CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4+and CD8+T cells. In addition, TNFSF14 / LIGHT / CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14 / LIGHT / CD258 is being actively investigated as a possible basis for cancer treatment.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy