Name: Granzyme B/GZMB Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-00817
Price: 697 CNY
Availability: InStock
Rating: 5 (10 reviews)

规格

价格

库存

数量

5 μg

¥ 697

现货

10 μg

¥ 1,150

现货

20 μg

¥ 1,890

3日内发货

50 μg

¥ 3,820

现货

100 μg

¥ 6,530

5日内发货

200 μg

¥ 11,100

5日内发货

500 μg

¥ 22,500

5日内发货

生物活性

产品描述	Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 3 substrates of GZMB have been identified including the key substrate caspase-3, ICAD, and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells and accordingly plays an essential role in immunosurveillance.
生物活性	Measured by its ability to cleave a peptide substrate, tButyloxycaronylAlaAlaAspThioBenzylester (BocAADSBzl),in the presence of 5,5’Dithiobis (2nitrobenzoic acid) (DTNB). The specific activity is >1500 pmol/min/μg. (The enzyme needs to be activated by Recombinant Mouse Active Cathepsin C)
研究背景	Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 3 substrates of GZMB have been identified including the key substrate caspase-3, ICAD, and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells and accordingly plays an essential role in immunosurveillance.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P10144
蛋白构建	A DNA sequence encoding the proform of human Granzyme B (NP_004122.1) (Met 1-Tyr 247) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Gly 19
蛋白纯度	≥ 97 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC.
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
别名	SECT, HLP, granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1), CTSGL1, CTLA1, CSP-B, CSPB, CGL-1, CGL1, CCPI
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.