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Ephrin A4/EFNA4 Protein, Human, Recombinant (His)

产品编号 TMPY-05444

Ephrin A4/EFNA4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 17.8 kDa and the accession number is P52798-1.

Ephrin A4/EFNA4 Protein, Human, Recombinant (His)

产品编号 TMPY-05444

Ephrin A4/EFNA4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 17.8 kDa and the accession number is P52798-1.

规格	价格	库存	数量
200 μg	¥ 4,420	5日内发货

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产品信息

生物活性	Measured by its binding ability in a functional ELISA. Immobilized recombinant Human EFNA4h(NEW) at 10 μg/ml(100 μl/well) can bind human EPHA7-His/Biotin , The EC50 of human EPHA7-His/Biotin is 150-320 ng/mL.
产品描述	Ephrin A4/EFNA4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 17.8 kDa and the accession number is P52798-1.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P52798-1
别名	LERK4,EPLG4,ephrin-A4,EFL4
蛋白构建	A DNA sequence encoding the human EFNA4 (NP_005218.1) (Met1-Gly171) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 26
蛋白纯度	> 95 % as determined by SDS-PAGE.
分子量	17.8 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	EPH-related receptor tyrosine kinase ligand 4 (Ephrin-A4) also known as EFNA4, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A4/EFNA4 functions as a cell surface GPI-bound ligand for Eph receptor, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development.