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Ephrin A3/EFNA3 Protein, Mouse, Recombinant (His)

产品编号 TMPY-01745

Ephrin A3/EFNA3 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O08545.

Ephrin A3/EFNA3 Protein, Mouse, Recombinant (His)

产品编号 TMPY-01745

Ephrin A3/EFNA3 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O08545.

规格	价格	库存	数量
100 μg	¥ 3,430	5日内发货

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产品信息

生物活性	Measured by its binding ability in a functional ELISA. Immobilized mouse EphrinA3 at 1 μg/ml (100 μl/well) can bind mouse EPHA6 with a linear range of 6.25-400 ng/ml.
产品描述	Ephrin A3/EFNA3 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O08545.
种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	O08545
别名	LERK-3,Epl3,ephrin-A3,Ehk1-L,EFL-2,AW494418
蛋白构建	A DNA sequence encoding the mouse EFNA3 (NP_034238.1) without the pro peptide (Met 1-Ser 205) was expressed, with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 23
蛋白纯度	> 92 % as determined by SDS-PAGE
分子量	22.2 kDa (predicted); 38 kDa (reducing condition, due to glycosylation)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Ephrin-A3 also known as EPH-related receptor tyrosine kinase ligand 3 or EFNA3, is a member of the ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A3 and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: Ephrin-A3 ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A3 expressed on astrocytes activates EphA4 on the post-synaptic neuron and restricts the growth of dendritic spines through multiple pathways.