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Enterokinase Protein, Bovine, Recombinant (Animal-Free, His)

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货号 TMPS-00026

别名 PRSS7, ENTK, Enteropeptidase

Enterokinase (EK) is a serine protease produced in the duodenum and involved in mammalian digestion. It activates trypsinogen to trypsin, thereby indirectly initiating the activation of pancreatic digestive enzymes. EK specifically cleaves after the recognition sequence Asp-Asp-Asp-Asp-Lys, but is inactive if a proline follows the cleavage site.
This recombinant bovine enterokinase is the light chain catalytic subunit, comprising a single glycosylated polypeptide chain corresponding to residues Ile801–His1035 of the native protein (Accession # P98072). Expressed in Pichia pastoris under animal-free conditions, the enzyme is highly active and suitable for applications in drug and vaccine development. A C-terminal 6×His tag is included to allow efficient removal by Ni²⁺ affinity chromatography following cleavage reactions.

Enterokinase Protein, Bovine, Recombinant (Animal-Free, His)

Enterokinase Protein, Bovine, Recombinant (Animal-Free, His)

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货号 TMPS-00026 别名 PRSS7, ENTK, Enteropeptidase

Enterokinase (EK) is a serine protease produced in the duodenum and involved in mammalian digestion. It activates trypsinogen to trypsin, thereby indirectly initiating the activation of pancreatic digestive enzymes. EK specifically cleaves after the recognition sequence Asp-Asp-Asp-Asp-Lys, but is inactive if a proline follows the cleavage site.
This recombinant bovine enterokinase is the light chain catalytic subunit, comprising a single glycosylated polypeptide chain corresponding to residues Ile801–His1035 of the native protein (Accession # P98072). Expressed in Pichia pastoris under animal-free conditions, the enzyme is highly active and suitable for applications in drug and vaccine development. A C-terminal 6×His tag is included to allow efficient removal by Ni²⁺ affinity chromatography following cleavage reactions.

规格价格库存数量
100 U
¥ 863
5日内发货
500 U
¥ 2,300
5日内发货
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产品介绍

生物活性
产品描述
Enterokinase (EK) is a serine protease produced in the duodenum and involved in mammalian digestion. It activates trypsinogen to trypsin, thereby indirectly initiating the activation of pancreatic digestive enzymes. EK specifically cleaves after the recognition sequence Asp-Asp-Asp-Asp-Lys, but is inactive if a proline follows the cleavage site.
This recombinant bovine enterokinase is the light chain catalytic subunit, comprising a single glycosylated polypeptide chain corresponding to residues Ile801–His1035 of the native protein (Accession # P98072). Expressed in Pichia pastoris under animal-free conditions, the enzyme is highly active and suitable for applications in drug and vaccine development. A C-terminal 6×His tag is included to allow efficient removal by Ni²⁺ affinity chromatography following cleavage reactions.
生物活性
5 U/μl Unit definition: One unit is defined as the amount of enzyme sufficient to cleave 50 μg of control fusion protein (~24 kDa) in 16 hours to 95% completion at 22 °C in a buffer containing 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl 2 , pH 7.4.
研究背景
Enterokinase (EK) is a serine protease produced in the duodenum and involved in mammalian digestion. It activates trypsinogen to trypsin, thereby indirectly initiating the activation of pancreatic digestive enzymes. EK specifically cleaves after the recognition sequence Asp-Asp-Asp-Asp-Lys, but is inactive if a proline follows the cleavage site.
种属
Bovine
标签His
蛋白纯度
≥ 95% as determined by SDS-PAGE
缓冲液Supplied as solution in 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, 50% glycerol, pH 7.4.
别名PRSS7, ENTK, Enteropeptidase
内毒素< 1.0 EU/μg of protein as determined by the LAL method.
化学信息
分子量40 kDa (Reducing conditions)
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储Upon receiving, the product remains stable for up to 6 months at -20 °C. This product is stable for up to 1 week at 37 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C.

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剂量转换

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技术支持

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