CD64 Protein, Mouse, Recombinant (His & Avi), Biotinylated

CD64 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 34.3 kDa and the accession number is P26151.

规格	价格/CNY		货期	数量
20 μg	￥ 2,940	5日内发货
100 μg	￥ 6,800	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 2,940

5日内发货

100 μg

￥ 6,800

5日内发货

生物活性

1. Immobilized Rituximab (IgG1) at 1 μg/mL can bind Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag) , the EC50 is 20-50 ng/mL. 2.Loaded Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.66 µM as determined in a BLI assay. 3.Loaded Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag) on SA Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 1.9 µM as determined in a BLI assay. 4. The purity of Mouse CD64 / FCGR1 Protein (His & AVI Tag), Biotinylated is more than 95% and the molecular weight of this protein is around 41-51 kDa verified by SEC-MALS.

产品描述

CD64 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 34.3 kDa and the accession number is P26151.

种属	Mouse
表达系统	HEK293 Cells
标签	His, Avi
蛋白编号	P26151
别名	Fc receptor, IgG, high affinity I, FcgammaRI, CD64, IGGHAFC, AI323638, FcγRI, AV092959
蛋白构建	The extracellular domain (Met 1-Pro 297) of Mouse FCGR1 (NP_034316.1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
蛋白纯度	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	34.3 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.