购物车

全部删除
您的购物车当前为空

CD64 Protein, Human, Recombinant (His)

产品编号 TMPY-04261

CD64 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 32 kDa and the accession number is P12314-1.

CD64 Protein, Human, Recombinant (His)

一键复制产品信息

产品编号 TMPY-04261

CD64 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 32 kDa and the accession number is P12314-1.

规格	价格	库存
5 μg	¥ 327	6-8日内发货
10 μg	¥ 518	6-8日内发货
20 μg	¥ 849	5日内发货
50 μg	¥ 1,660	5日内发货
100 μg	¥ 2,790	In stock
200 μg	¥ 4,930	5日内发货
500 μg	¥ 10,470	5日内发货

大包装 & 定制

加入购物车

TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

实验操作小课堂

常见问题解答

选择批次

资源下载

COA 重组蛋白用户指南

产品信息

生物活性	1. Immobilized Anti-CD20(Ro) Antibody (Rituximab) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD64/FCGR1A Protein (His Tag), the EC50 is 0.6-2.0 ng/mL. 2. Captured Human FcγRI/CD64 recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 5.5 nM as determined in an SPR assay. 3. Captured Human FcγRI/CD64 recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 13.6 nM as determined in an SPR assay. 4. Loaded Human FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 8.5 nM as determined in a BLI assay. 5. Loaded Human FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 4.7 nM as determined in a BLI assay. 6. Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRI/CD64 recombinant protein with an affinity constant of 0.38 nM as determined in a BLI assay. 7. The purity of Human CD64/FCGR1A Protein (His Tag) is more than 95% and the molecular weight of this protein is around 38-52 kDa verified by SEC-MALS.
产品描述	CD64 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 32 kDa and the accession number is P12314-1.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P12314-1
别名	Fc γ RI,Fc receptor, IgG, high affinity I,Fc gamma RI,CD64
蛋白构建	A DNA sequence encoding the extracellular domain of human CD64A (NP_000557.1) (Met 1-Pro 288) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 16
蛋白纯度	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
分子量	32 kDa (predicted); 45-60 kDa (reducing condition, due to glycosylation)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.