Carbonic Anhydrase 8 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 33.8 kDa and the accession number is P35219.

Human

E. coli

MGC99509,MGC120502,CA-VIII,CARP,carbonic anhydrase VIII,CAMRQ3,CALS

A DNA sequence encoding the human CA8 (NP_004047.3) (Met 1-Gln 290) was expressed, with a polyhistidine tag at the C-terminus. Predicted N terminal: Met 1

> 94 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.