Lipopolysaccharides from E. coli O127:B8 (LPS, from Escherichia coli (O127:B8)) are endotoxins and TLR-4 activators extracted from the bacterium E. coli O127:B8. This smooth (S) type LPS features a typical tripartite structure: an O antigen, an R3 core oligosaccharide, and lipid A. When lipopolysaccharides from E. coli O127:B8 activate TLR-4 on immune cells, they can trigger inflammatory responses and ileal contractility, making them useful for developing intestinal inflammation models.