Anti-Sulfiredoxin-1 Polyclonal Antibody is a Rabbit antibody targeting Sulfiredoxin-1. Anti-Sulfiredoxin-1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Sample: Kidney (Mouse) Lysate at 40 μg
Primary: Anti-SRXN1 (TMAB-01797) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
2. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Rat) Lysate at 40 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-SRXN1 (TMAB-01797) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RTfor 30 min; Antibody incubation with (SRXN1) Polyclonal/MonoclonalAntibody, Unconjugated (TMAB-01797) at 1:400 overnight at 4°C,followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200

Polyclonal

KLH conjugated synthetic peptide: human SRXN1

Human

SRXN1

Sulfiredoxin-1

Oxidative Stress,Oxidative stress

SRXN1 contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. It does not act on PRDX5 or PRDX6. SRXN1 may catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase.