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Anti-Sulfiredoxin-1 Polyclonal Antibody
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产品编号 TMAB-01797
别名 Sulfiredoxin-1, SRXN1, SRX1, SRX, C20orf139
Anti-Sulfiredoxin-1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Sulfiredoxin-1。Anti-Sulfiredoxin-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Sulfiredoxin-1 Polyclonal Antibody
Anti-Sulfiredoxin-1 Polyclonal Antibody
还可以产品编号 TMAB-01797 别名 Sulfiredoxin-1, SRXN1, SRX1, SRX, C20orf139
Anti-Sulfiredoxin-1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Sulfiredoxin-1。Anti-Sulfiredoxin-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Sulfiredoxin-1 Polyclonal Antibody is a Rabbit antibody targeting Sulfiredoxin-1. Anti-Sulfiredoxin-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| 别名 | Sulfiredoxin-1, SRXN1, SRX1, SRX, C20orf139 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| 验证活性 | 1. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-SRXN1 (TMAB-01797) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa 2. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Rat) Lysate at 40 μg Lane 3: HepG2 (Human) Cell Lysate at 30 μg Lane 4: A549 (Human) Cell Lysate at 30 μg Primary: Anti-SRXN1 (TMAB-01797) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RTfor 30 min; Antibody incubation with (SRXN1) Polyclonal/MonoclonalAntibody, Unconjugated (TMAB-01797) at 1:400 overnight at 4°C,followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasmic |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Sulfiredoxin, also designated Sulfiredoxin-1 and chromosome 20 open reading frame 139 (C20orf139), is a cytoplasmic antioxidant protein involved in signaling through catalytic reduction of oxidative modifications. It regulates peroxiredoxins (PRXs), a family of proteins that reduce hydroperoxides, by reducing the conserved cysteine from sulfinic to sulfenic acid. This impacts the role of PRX in the reduction of other downstream transcription factors and kinase signaling pathways. The Sulfiredoxin protein specifically acts on the PRDX1, PRDX2, PRDX3 and PRDX4 peroxiredoxins, but not on PRDX5 or PRDX6. Sulfiredoxin acts as a phosphotransferase and an athioltransferase and is widely expressed, with highest levels detected in lung, spleen, kidney and thymus tissues. |
| 免疫原 | KLH conjugated synthetic peptide: human SRXN1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Oxidative Stress,Oxidative stress |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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