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Anti-PRKG1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 PRKG1。Anti-PRKG1 Polyclonal Antibody 可用于 WB,ICC/IF。
Anti-PRKG1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 PRKG1。Anti-PRKG1 Polyclonal Antibody 可用于 WB,ICC/IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,785 | 5日内发货 |
产品描述 | Anti-PRKG1 Polyclonal Antibody is a Rabbit antibody targeting PRKG1. Anti-PRKG1 Polyclonal Antibody can be used in WB,ICC/IF. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
验证活性 | 1. Sample: Hela Cell Lysate at 40 μg U2OS Cell Lysate at 40 μg Primary: Anti-cGKI (TMAB-01572) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 74 kDa 2. Sample: Heart (Mouse) Lysate at 40 μg Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-cGKI (TMAB-01572) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 74 kDa 3. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (cGKI) polyclonal Antibody, Unconjugated (TMAB-01572) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
应用 | |
推荐剂量 | WB: 1:500-2000; ICC/IF: 1:100 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cytoplasm. Colocalized with TRPC7 in the plasma membrane. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. PKG plays an important stimulatory role in platelet activation. |
免疫原 | KLH conjugated synthetic peptide: human Prkg1/cGKI |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Platelets,Hypertrophy,Other Kinases |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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