Anti-PARP Antibody (1W26)

Name: Anti-PARP Antibody (1W26)
Brand: TargetMol
SKU: TMAY-01509
Rating: 4.5 (1 reviews)

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产品编号 TMAY-01509

Anti-PARP Antibody (1W26) 是一种 Mouse 抗体，靶向 PARP。Anti-PARP Antibody (1W26) 可用于 WB,IHC-P,IP。

Anti-PARP Antibody (1W26)

还可以

产品编号 TMAY-01509

Anti-PARP Antibody (1W26) 是一种 Mouse 抗体，靶向 PARP。Anti-PARP Antibody (1W26) 可用于 WB,IHC-P,IP。

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生物活性

产品描述	Anti-PARP Antibody (1W26) is a Mouse antibody targeting PARP. Anti-PARP Antibody (1W26) can be used in WB,IHC-P,IP.
Ig Type	Monoclonal Mouse IgG1
克隆号	1W26
交叉反应	Human
验证活性	1. Immunochemical staining of human PARP1 in human placenta with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 2. Immunochemical staining of human PARP1 in human lymph node with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 3. Immunochemical staining of human PARP1 in human breast with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 4. Immunochemical staining of human PARP1 in human brain with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 5. PARP1 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate -0.5 µL anti-PARP1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PARP1 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 113 kDa. -Observed band size: 113 kDa. 6. Anti-PARP1 mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:113 kDa. -Observed band size:113 kDa
应用	WB,IHC-P,IP
推荐剂量	WB: 1:500-1:1000; IHC-P: 1:100-1:500; IP: 0.2-1 μL/mg of lysate
抗体种类	Monoclonal
宿主来源	Mouse
构建方式	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PARP-1 / PARP (rh PARP-1 / PARP; TMPY-01188; NP_001609.2; Met 1-Trp 1014). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式	Protein A
性状	Liquid
缓冲液	0.2 μm filtered solution in PBS
研究背景	Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrated to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors are thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	Recombinant Human PARP-1 / PARP protein (TMPY-01188)
抗原种属	Human
研究领域	Cancer Drug Targets

存储&运输

储存方式	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式	Shipping with blue ice.