Anti-p53 Antibody (9G129)

Name: Anti-p53 Antibody (9G129)
Brand: TargetMol
SKU: TMAY-03856
Rating: 4.5 (1 reviews)

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产品编号 TMAY-03856

Anti-p53 Antibody (9G129) 是一种 Rabbit 抗体，靶向 p53。Anti-p53 Antibody (9G129) 可用于 IHC-P,ICC/IF。

Anti-p53 Antibody (9G129)

还可以

产品编号 TMAY-03856

Anti-p53 Antibody (9G129) 是一种 Rabbit 抗体，靶向 p53。Anti-p53 Antibody (9G129) 可用于 IHC-P,ICC/IF。

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生物活性

产品描述	Anti-p53 Antibody (9G129) is a Rabbit antibody targeting p53. Anti-p53 Antibody (9G129) can be used in IHC-P,ICC/IF.
Ig Type	Monoclonal Rabbit IgG
克隆号	9G129
交叉反应	Cynomolgus, Human
验证活性	1. Immunofluorescence staining of p53 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Cynomolgus p53 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus. 2. Immunochemical staining of p53 in human lung cancer with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 3. Immunochemical staining of p53 in human gastric cancer with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).
应用	IHC-P,ICC/IF
推荐剂量	IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100
抗体种类	Monoclonal
宿主来源	Rabbit
构建方式	This antibody was obtained from a rabbit immunized with purified, recombinant Cynomolgus p53 / TP53 (TMPY-02228; E3U906; Met1-Asp393).
纯化方式	Protein A
性状	Liquid
缓冲液	0.2 μm filtered solution in PBS
研究背景	p53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitin ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	Recombinant Cynomolgus p53 / TP53 protein (TMPY-02228)
抗原种属	Cynomolgus
研究领域	Apoptosis Transcription Factors and Regulators, Cancer Drug Targets, Tumor Suppressors

存储&运输

储存方式	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式	Shipping with blue ice.