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Anti-GRIA1 Polyclonal Antibody

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Anti-GRIA1 Polyclonal Antibody
产品编号 TMAB-00810

Anti-GRIA1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 GRIA1。Anti-GRIA1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,IP。

Anti-GRIA1 Polyclonal Antibody

Anti-GRIA1 Polyclonal Antibody

Rating icon 还可以
Anti-GRIA1 Polyclonal Antibody
产品编号 TMAB-00810

Anti-GRIA1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 GRIA1。Anti-GRIA1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,IP。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-GRIA1 Polyclonal Antibody is a Rabbit antibody targeting GRIA1. Anti-GRIA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,IP.
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Paraformaldehyde-fixed, paraffin embedded Mouse hippocampal; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat hippocampal; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. (Negative control) paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GluR1 Polyclonal Antibody, Unconjugated (TMAB-00810) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. 25 μg total protein per Lane of various lysates probed with GluR1 polyclonal antibody, unconjugated (TMAB-00810) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
应用
推荐剂量
WB: 1:500-1:2000; IHC-P: 1:100-1:200; IHC-Fr: 1:100-1:200; IF: 1:100-1:200; IP: 1:20-1:50
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendrit. Cell projection, dendritic spine. Note=Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
抗原信息
免疫原
KLH conjugated synthetic peptide: human GluR1
抗原种属
Human
基因ID
Uniprot ID
研究领域
Glutamate Receptors,AMPA / Kainate
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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