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Anti-GPX4 Polyclonal Antibody
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产品编号 TMAB-00806
Anti-GPX4 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 GPX4。Anti-GPX4 Polyclonal Antibody 可用于 WB。
Anti-GPX4 Polyclonal Antibody
Anti-GPX4 Polyclonal Antibody
还可以产品编号 TMAB-00806
Anti-GPX4 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 GPX4。Anti-GPX4 Polyclonal Antibody 可用于 WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-GPX4 Polyclonal Antibody is a Rabbit antibody targeting GPX4. Anti-GPX4 Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Pig,Cow,Sheep) |
| 验证活性 | 1. Sample: Kidney (mouse) Lysate at 40 μg Primary: Anti-GPX4 (TMAB-00806) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 20 kDa 2. Sample: Lane 1: Testis (Mouse) Tissue Lysate at 40 μg Lane 2: Liver (Mouse) Tissue Lysate at 40 μg Lane 3: Kidney (Mouse) Tissue Lysate at 40 μg Lane 4: Heart (Mouse) Tissue Lysate at 40 μg Lane 5: Testis (Rat) Tissue Lysate at 40 μg Lane 6: Liver (Rat) Tissue Lysate at 40 μg Lane 7: Kidney (Rat) Tissue Lysate at 40 μg Lane 8: Heart (Rat) Tissue Lysate at 40 μg Lane 9: U937 (Human) Cell Lysate at 30 μg Lane 10: HepG2 (Human) Cell Lysate at 30 μg Lane 11: MCF-7 (Human) Cell Lysate at 30 μg Lane 12: Huvec (Human) Cell Lysate at 30 μg Lane 13: 293T (Human) Cell Lysate at 30 μg Primary: Anti-GPX4 (TMAB-00806) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 20-22 kDa 3. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-GPX4 (TMAB-00806) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 19 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site. |
| 免疫原 | KLH conjugated synthetic peptide: human Glutathione Peroxidase 4 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cellular metabolic process,Mitochondrial markers,Antioxidants,Cancer,Mitochondrial |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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