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Anti-DLG4 Polyclonal Antibody 2

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Anti-DLG4 Polyclonal Antibody 2
产品编号 TMAB-00552

Anti-DLG4 Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 DLG4。Anti-DLG4 Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-DLG4 Polyclonal Antibody 2

Anti-DLG4 Polyclonal Antibody 2

Rating icon 还可以
Anti-DLG4 Polyclonal Antibody 2
产品编号 TMAB-00552

Anti-DLG4 Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 DLG4。Anti-DLG4 Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-DLG4 Polyclonal Antibody 2 is a Rabbit antibody targeting DLG4. Anti-DLG4 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit,Sheep)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PSD95) Polyclonal Antibody, Unconjugated (TMAB-00552) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PSD95) Polyclonal Antibody, Unconjugated (TMAB-00552) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PSD95) Polyclonal Antibody, Unconjugated (TMAB-00552) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PSD95) Polyclonal Antibody, Unconjugated (TMAB-00552) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Sample:
Lane 1: Spinal cord (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Rat) Lysate at 40 μg
Lane 3: Cerebrum (Mouse) Lysate at 40 μg
Lane 4: Cerebellum (Rat) Lysate at 40 μg
Lane 5: Cerebellum (Mouse) Lysate at 40 μg
Primary: Anti-PSD95 (TMAB-00552) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 95 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Cell junction, synapse, synaptosome. Note=High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
抗原信息
免疫原
KLH conjugated synthetic peptide: human PSD95
抗原种属
Human
基因ID
Uniprot ID
研究领域
Adapters,Synapse marker
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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