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PD-1 Protein, Cynomolgus, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.4 kDa and the accession number is B0LAJ3.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
5 μg | ¥ 428 | 6-8日内发货 | |
10 μg | ¥ 683 | 6-8日内发货 | |
20 μg | ¥ 1,130 | 5日内发货 | |
50 μg | ¥ 2,270 | 5日内发货 | |
100 μg | ¥ 4,430 | 5日内发货 | |
200 μg | ¥ 6,730 | 5日内发货 | |
500 μg | ¥ 13,800 | 5日内发货 | |
1 mg | ¥ 28,800 | 5日内发货 |
生物活性 | Immobilized Cynomolgus PD-1, hFc Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 29.5 ng/ml determined by ELISA (QC Test).Cynomolgus PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus PD-L1, His Tag with an affinity constant of 1.28 μM as determined in SPR assay (Biacore T200). |
产品描述 | PD-1 Protein, Cynomolgus, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.4 kDa and the accession number is B0LAJ3. |
种属 | Cynomolgus |
表达系统 | HEK293 Cells |
标签 | C-hFc |
蛋白编号 | B0LAJ3 |
别名 | programmed cell death 1 |
蛋白构建 | Pro21-Gln167 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 42.4 kDa (Predicted); 55-70 kDa (Due to glycosylation) |
内毒素 | Less than 1EU per μg by the LAL method. |
缓冲液 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity. |