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Donkey Anti-Rabbit IgG H&L Secondary Antibody-Cy5 是一种 Cy5-conjugated Donkey 抗体,靶向 IgG H&L。Donkey Anti-Rabbit IgG H&L Secondary Antibody-Cy5 可用于 IF,FCM,ICC/IF。
Donkey Anti-Rabbit IgG H&L Secondary Antibody-Cy5 是一种 Cy5-conjugated Donkey 抗体,靶向 IgG H&L。Donkey Anti-Rabbit IgG H&L Secondary Antibody-Cy5 可用于 IF,FCM,ICC/IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μL | ¥ 355 | 5日内发货 | |
1 mL | ¥ 2,385 | 5日内发货 |
产品描述 | Donkey Anti-Rabbit IgG H&L Secondary Antibody-Cy5 is a Cy5-conjugated Donkey antibody targeting IgG H&L. Donkey Anti-Rabbit IgG H&L Secondary Antibody-Cy5 can be used in IF,FCM,ICC/IF. |
别名 | Immunoglobulin G, Donkey Anti-Rabbit IgG H&L (Cy5) |
Ig Type | IgG |
交叉反应 | Rabbit |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei. 2. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei. 3. Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei. 5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei. 6. Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei. 7. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei. 8. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei. 9. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugatedat 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei. 10. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei. ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
应用 | |
推荐剂量 | IF: 1:100-1000; FCM: 1:100-1000; ICC/IF: 1:100-1000 |
抗体种类 | Polyclonal |
宿主来源 | Donkey |
构建方式 | Polyclonal Antibody |
纯化方式 | Protein G purified |
性状 | Liquid |
缓冲液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
浓度 | 2.0 mg/ml |
研究背景 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
免疫原 | Native Rabbit IgG |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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