Anti-Mucin-4/MUC4 Polyclonal Antibody is a Rabbit antibody targeting Mucin-4/MUC4. Anti-Mucin-4/MUC4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Pig,Cow,Horse,GuineaPig)

1. Sample:
Panc-1 (Human) Cell Lysate at 30 μg
LOVO (Human) Cell Lysate at 30 μg
Thymus (Mouse) Lysate at 40 μg
MKN45 (Human) Cell Lysate at 30 μg
Primary: Anti-MUC4 (TMAB-01187) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 120 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Human lung carcinom); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Muc4) Polyclonal Antibody, Unconjugated (TMAB-01187) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human oral squamous cell carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Muc4) Polyclonal Antibody, Unconjugated (TMAB-01187) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Mucin-4 beta chain

Human

MUC,Tumor antigens,Tumor-associated antigens,Defensin,MUC