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B7-1 Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPY-06298

B7-1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 27.12 kDa and the accession number is A0N0P2.

B7-1 Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPY-06298

B7-1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 27.12 kDa and the accession number is A0N0P2.

规格	价格	库存	数量
20 μg	¥ 2,940	5日内发货
100 μg	¥ 6,800	5日内发货

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产品信息

生物活性	Measured by its binding ability in a functional ELISA. Immobilized Human CTLA-4 hFc at 2 μg/mL (100 μL/well) can bind Human B7-1, Biotinylated, the EC50 of Human B7-1, Biotinylated is 60-320 ng/mL.
产品描述	B7-1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 27.12 kDa and the accession number is A0N0P2.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	A0N0P2
别名	LAB7,CD80 molecule,CD28LG1,CD28LG,BB1,B7-1,B7.1,B7
蛋白构建	A DNA sequence encoding the human CD80 (NP_005182.1)(Met1-Asn242) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Val 35
蛋白纯度	> 95 % as determined by SDS-PAGE.
分子量	27.12 kDa (predicted); 48.86 kDa (reducing conditions)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy