Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development. The administration of PDGFB alone normalized tumor vasculature by increasing periendothelial coverage and vascular functionality. Interestingly, this effect exerted by PDGFB was also observed in the presence of DAPT. So PDGFB is able to improve tumor vascularity and allows the anticancer action of DAPT in the tumor. PDGFB Protein, Cynomolgus, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 38.9 kDa and the accession number is G7PFK7.
生物活性 | 1. Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 5-25 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus PDGFRB-His at 10 μg/ml (100 μl/well) can bind Cynomolgus S4-mFc3-PDGFB, The EC50 of Cynomolgus S4-mFc3-PDGFB is 3.6-7.4 ng/ml. |
产品描述 | Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development. The administration of PDGFB alone normalized tumor vasculature by increasing periendothelial coverage and vascular functionality. Interestingly, this effect exerted by PDGFB was also observed in the presence of DAPT. So PDGFB is able to improve tumor vascularity and allows the anticancer action of DAPT in the tumor. PDGFB Protein, Cynomolgus, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 38.9 kDa and the accession number is G7PFK7. |
种属 | Cynomolgus |
表达系统 | HEK293 Cells |
标签 | N-mFc |
蛋白编号 | G7PFK7 |
别名 | 血小板源生长因子, platelet-derived growth factor beta polypeptide, platelet-derived growth factor β polypeptide |
蛋白构建 | The cynomolgus PDGFB (EHH65851.1) (Ser82-Thr190) was expressed with the Fc region of Mouse IgG1 at the N-terminus. |
蛋白纯度 | > 95 % as determined by SDS-PAGE |
分子量 | 38.9 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development. The administration of PDGFB alone normalized tumor vasculature by increasing periendothelial coverage and vascular functionality. Interestingly, this effect exerted by PDGFB was also observed in the presence of DAPT. So PDGFB is able to improve tumor vascularity and allows the anticancer action of DAPT in the tumor. |
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PDGFB Protein, Cynomolgus, Recombinant (mFc) 血小板源生长因子 platelet-derived growth factor beta polypeptide platelet-derived growth factor β polypeptide platelet-derived growth factor b polypeptide recombinant recombinant-proteins proteins protein