Powder: -20°C for 3 years | In solvent: -80°C for 1 year
MT 63-78 是一种有效的直接AMPK 激活剂,EC50为 25 μM。它诱导细胞有丝分裂阻滞和细胞凋亡,通过抑制脂肪生成和mTORC1途径来阻止前列腺癌的生长,具有抗肿瘤作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 663 | 现货 | ||
2 mg | ¥ 992 | 现货 | ||
5 mg | ¥ 1,590 | 现货 | ||
10 mg | ¥ 2,670 | 现货 | ||
25 mg | ¥ 4,480 | 现货 | ||
50 mg | ¥ 6,380 | 现货 | ||
100 mg | ¥ 8,730 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,630 | 现货 |
产品描述 | MT 63-78 is a specific and effective direct AMPK activator (EC50: 25 μM). MT 63-78 blocks prostate cancer growth by inhibiting the lipogenesis and mTORC1 pathways. MT 63-78 has antitumor effects. MT 63–78 also causes cell mitotic arrest and apoptosis. |
靶点活性 | mTORC1:, AMPK:25 μM (EC50) |
体外活性 | MT 63-78 (0-50 μM; 24 hours; LNCaP, PC3, C4-4, C4-2B, CL1and 22RV1cells) treatment induces reduction of anti-apoptotic Mcl-1 in concert with an accumulation of the pro-apoptotic BH3-only protein Puma. MT 63-78 (0-50 μM; 4 days; LNCaP and PC3 cells) treatment displays a dose-dependent decrease in cell number and concomitant to the activation of AMPK signaling. MT 63-78 (0-50 μM; 30 minutes; LNCaP and PC3 cells) treatment shows dose-dependent phosphorylation of the two major AMPK targets Acetyl-CoA Carboxylase (ACC) on Ser79 and of Raptor on Ser792. And also increases Thr172 phosphorylation on the AMPK α subunit. MT 63-78 (25 μM; 24 hours; LNCaP and CRPC cells) treatment causes a significant enrichment in the G2/M population . |
体内活性 | MT 63-78 (30 mg/kg; intraperitoneal injection; daily; for 14 days; C57 BL/6 male mice) treatment causes a 33% inhibition of tumor growth. |
分子量 | 326.35 |
分子式 | C21H14N2O2 |
CAS No. | 1179347-65-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 3.26 mg/mL (10 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.0642 mL | 15.321 mL | 30.6419 mL | 76.6049 mL |
5 mM | 0.6128 mL | 3.0642 mL | 6.1284 mL | 15.321 mL | |
10 mM | 0.3064 mL | 1.5321 mL | 3.0642 mL | 7.6605 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
MT 63-78 1179347-65-9 Apoptosis Chromatin/Epigenetic PI3K/Akt/mTOR signaling AMPK mTOR Raptor mitotic Mammalian target of Rapamycin direct antitumor Thr172 arrest dephosphorylation Inhibitor AMP-activated protein kinase Mcl-1 mTORC1 MT 63 78 lipogenesis BH3 MT 6378 p53 inhibit inhibitor