Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Lucanthone (Lucanthonum) 是一种 Apurinic 核酸内切酶-1 的抑制剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 428 | 现货 | ||
2 mg | ¥ 623 | 现货 | ||
5 mg | ¥ 1,060 | 现货 | ||
10 mg | ¥ 1,690 | 现货 | ||
25 mg | ¥ 2,960 | 现货 | ||
50 mg | ¥ 4,370 | 现货 | ||
100 mg | ¥ 6,170 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,170 | 现货 |
产品描述 | Lucanthone (Lucanthonum) is an inhibitor of Apurinic endonuclease-1 (APE-1). |
体外活性 | Lucanthone is a novel inhibitor of autophagy that induces cathepsin D-mediated apoptosis. Cell viability is measured by MTT assay to investigate the anticancer activity of Lucanthone. Lucanthone reduces cell viability to a similar extent in a panel of seven breast cancer cell lines. In addition, a direct comparison reveals that Lucanthone is significantly more potent than Chloroquine (CQ) at reducing breast cancer cell viability with a mean IC50 of 7.2 μM versus 66 μM for CQ. Measurement of cell viability in two representative cell lines (MDA-MB-231 and BT-20) by ATPlite assay and trypan blue exclusion reveals comparable results[2]. |
细胞实验 | Cell viability is assessed by MTT assay. Cells are seeded into 96-well microculture plates at 10,000 cells per well and allowed to attach for 24 h. Cells are then treated with Lucanthone (0, 0.5, 1, 5, 10, 20 and 40 μM), Chloroquine, Vorinostat, or combinations for 72 h. Following drug treatment, MTT is added and cell viability is quantified using a BioTek microplate reader. Effects on cell viability are also determined by measuring ATP levels using the ATPlite assay system and by trypan blue exclusion. Pro-apoptotic effects following in vitro drug exposure are quantified by propidium iodide (PI) staining and fluorescence-activated cell sorting (FACS) analysis of sub-G0/G1 DNA content[2] |
别名 | Lucantona, Lucanthonum, 硫坎酮, 硫蒽酮 |
分子量 | 340.48 |
分子式 | C20H24N2OS |
CAS No. | 479-50-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/mL (176.22 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.937 mL | 14.6852 mL | 29.3703 mL | 73.4258 mL |
5 mM | 0.5874 mL | 2.937 mL | 5.8741 mL | 14.6852 mL | |
10 mM | 0.2937 mL | 1.4685 mL | 2.937 mL | 7.3426 mL | |
20 mM | 0.1469 mL | 0.7343 mL | 1.4685 mL | 3.6713 mL | |
50 mM | 0.0587 mL | 0.2937 mL | 0.5874 mL | 1.4685 mL | |
100 mM | 0.0294 mL | 0.1469 mL | 0.2937 mL | 0.7343 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Lucanthone 479-50-5 Autophagy Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis Lucantona inhibit Lucanthonum 硫坎酮 Inhibitor 硫蒽酮 inhibitor