Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Lorcaserin HCl 是一种选择性血清素激动剂,被批准为减肥药。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 142 | 现货 | ||
5 mg | ¥ 287 | 现货 | ||
10 mg | ¥ 407 | 现货 | ||
25 mg | ¥ 665 | 现货 | ||
50 mg | ¥ 981 | 现货 | ||
100 mg | ¥ 1,460 | 现货 | ||
500 mg | ¥ 3,550 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 387 | 现货 |
产品描述 | Lorcaserin is a selective serotonin agonist that has been recently approved as a weight loss agent. |
体外活性 | Lorcaserin is 18-fold more potent at human 5-HT2C (EC50= 9 nM) than at human 5-HT2A (EC50= 168 nM), and it is 104-fold more potent at human 5-HT2C than human 5-HT2B (EC50= 943 nM) |
体内活性 | Lorcaserin decreases cumulative food intake at 2, 4, 6, and 22 h with a significant decrease continuing throughout the 22-h duration of the study at the highest dose (24 mg/kg) only. The reduction in food intake induced by lorcaserin (18 mg/kg, p.o.) is mediated by 5-HT2C and not 5-HT2A receptors. Lorcaserin significantly reduces active, and increased inactive counts, and increases the incidence of penile grooming, an effect mediated by the 5-HT2C receptor[1]. Lorcaserin (20 mg/kg) doses causes a significant linear dose-related reduction in responses on the active lever but has no significant effect on the response to the inactive lever. Lorcaserin (0.3125, 0.625, 1.25, and 2.5 mg/kg) also produces a significant reduction in nicotine self-administration[ |
细胞实验 | For development of functional human and rat 5-HT2A,5-HT2B,and 5-HT2C inositol accumulation assays,recombinant 5-HT2 receptors are transiently expressed in HEK293 cells using the Lipofectamine method.Cells are incubated in 15-cm plates with DMEM,and they are grown to at least 60 to 80% confluence,rinsed with Opti-MEM I media,followed by addition of 2 mL of Opti-MEM I containing 60 μL of Lipofectamine and 16 μg of cDNA for human and rat 5-HT2A and 5-HT2B receptors or 15 μL of Lipofectamine and 16 μg of cDNA for human and rat 5-HT2C receptors.Cells are then incubated for 5 h at 37°C in a 5% CO2 incubator.Next,cells are aspirated,and 30 mL of DMEM is added.Cells are then used for assays 24 h after transfection.Under these conditions,cells expressing human 5-HT2C receptor display constitutive elevation of inositol phosphate accumulation that is reversed by the inverse agonist mianserin,whereas cells expressing human 5-HT2A and 5-HT2B receptors display considerably less constitutive activity. |
动物实验 | On the day of the experiment, rats receives a single dose of lorcaserin in water via oral gavage. Dosing volume and dose are 5 mL/kg and 10 mg/kg, respectively. |
分子量 | 232.15 |
分子式 | C11H14ClN·HCl |
CAS No. | 846589-98-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 2.4 mg/mL (10.34 mM)
Ethanol: 46 mg/mL (198.14 mM)
H2O: 46 mg/mL (198.14 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol / H2O | 1 mM | 4.3076 mL | 21.5378 mL | 43.0756 mL | 107.689 mL |
5 mM | 0.8615 mL | 4.3076 mL | 8.6151 mL | 21.5378 mL | |
10 mM | 0.4308 mL | 2.1538 mL | 4.3076 mL | 10.7689 mL | |
Ethanol / H2O | 20 mM | 0.2154 mL | 1.0769 mL | 2.1538 mL | 5.3844 mL |
50 mM | 0.0862 mL | 0.4308 mL | 0.8615 mL | 2.1538 mL | |
100 mM | 0.0431 mL | 0.2154 mL | 0.4308 mL | 1.0769 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Lorcaserin HCl 846589-98-8 GPCR/G Protein Neuroscience 5-HT Receptor Inhibitor inhibitor inhibit