Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Dp44mT 是一种具有选择性抗癌活性的铁螯合剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 297 | 现货 | ||
10 mg | ¥ 493 | 现货 | ||
25 mg | ¥ 978 | 现货 | ||
50 mg | ¥ 1,560 | 现货 | ||
100 mg | ¥ 2,430 | 现货 | ||
200 mg | ¥ 3,620 | 现货 | ||
500 mg | ¥ 5,790 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 478 | 现货 |
产品描述 | Dp44mT, a effective iron chelator, has selective antitumor activity. |
体外活性 | In MDA-MB-231 cells, Dp44mT selectively targets towards topoisomerase topo2α resulting in DNA damage. Dp44mT inhibits antiproliferative effects significantly with IC50 of 30 nM, 60 nM, and 60 nM for SK-N-MC, SK-Mel-28, and MCF-7 cells, respectively, while showing no effects on normal MRC-5 fibroblasts. In SK-N-MC neuroepithelioma and M109 cells,Dp44mT inhibits cellular Fe uptake from Fe-Tf , and induces cell apoptosis. Dp44mT, as a Pgp substrate, also overcomes multidrug resistance by the hijacking of lysosomal P-glycoprotein (Pgp). |
体内活性 | Dp44mT(0.4 mg/kg,静脉注射)通过剂量依赖方式抑制CD2F1小鼠体内植入的M109肿瘤。 |
细胞实验 | SK-N-MC, SK-Mel-28, MCF-7 and MRC-5 cells are incubated in the presence and absence of DFO, 311, 3-aminopyridine-2-carboxaldehyde thiosemicarbazone, doxorubicin, and the DpT series of chelators (0-25 μM) for 72 hours at 37°C. The effect of the chelators on proliferation is examined using the MTT assay. |
动物实验 | CD2F1 mice that are implanted M109 tumors intravenous inject Dp44mT (dissolved in Propylene glycol) 4 mg/kg in twice daily. |
别名 | 2-(二-2-吡啶基亚甲基)-N,N-二甲基肼基硫代甲酰胺 |
分子量 | 285.37 |
分子式 | C14H15N5S |
CAS No. | 152095-12-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 57 mg/mL (199.74 mM)
Ethanol: 47 mg/mL (164.69 mM)
H2O: Insoluble
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 3.5042 mL | 17.5211 mL | 35.0422 mL | 87.6056 mL |
5 mM | 0.7008 mL | 3.5042 mL | 7.0084 mL | 17.5211 mL | |
10 mM | 0.3504 mL | 1.7521 mL | 3.5042 mL | 8.7606 mL | |
20 mM | 0.1752 mL | 0.8761 mL | 1.7521 mL | 4.3803 mL | |
50 mM | 0.0701 mL | 0.3504 mL | 0.7008 mL | 1.7521 mL | |
100 mM | 0.035 mL | 0.1752 mL | 0.3504 mL | 0.8761 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Dp44mT 152095-12-0 Apoptosis Others Ferroptosis 2-(二-2-吡啶基亚甲基)-N,N-二甲基肼基硫代甲酰胺 inhibit Dp-44mT Inhibitor inhibitor