Powder: -20°C for 3 years | In solvent: -80°C for 1 year
UNC 669是一种有效且特异性的 MBT(恶性脑瘤)抑制剂,是甲基赖氨酸结合结构域的配体,是L3MBTL1和L3MBTL3的抑制剂,IC50分别为 4.2 和 3.1 uM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 294 | 现货 | ||
10 mg | ¥ 493 | 现货 | ||
25 mg | ¥ 798 | 现货 | ||
50 mg | ¥ 1,136 | 现货 | ||
100 mg | ¥ 1,980 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 336 | 现货 |
产品描述 | UNC 669 is an effective and specific MBT (malignant brain tumor) inhibitor with IC50 of 4.2/3.1 uM for L3MBTL1/3. |
靶点活性 | L3MBTL1:4.2 μM, L3MBTL3:3.1 μM |
激酶实验 | In vitro Enzyme Assays.: All in vitro enzyme assays are done at Upstate Biotechnology with the exception of InsR and IGF-1R. To determine the IC50 of TAE684 against InsR and IGF-1R a homogeneous time-resolved fluorescence assay is performed. ATP (10 mM) and 20 mg/ml biotinylated PolyEY (Glu, Tyr 4:1) are combined with 50 nL of serial dilutions of TAE684 (10-500 nM) and 4 ng of InsR enzyme in the presence of the kinase reaction buffer (20 mM Tris譎Cl, pH 7.5/10 mM MgCl2/3 mM MnCl2/1 mM DTT/10 mM NaVO4/0.1 mg/ml of BSA). Assays are incubated for 1 hour at ambient temperature. Reactions are terminated by adding 10 mL of the detection solution containing 50 mM EDTA, 500 mM KF, 0.5 mg/ml of BSA, 5 mg/mL Eu3+ cryptate-labeled anti-phosphotyrosine antibody Mab PT66-K, and 5 mg/mL Streptavidin-XLent. The reaction is incubated for half an hour, and fluorescence signals are read on Analyst GT. |
别名 | UNC669 |
分子量 | 338.24 |
分子式 | C15H20BrN3O |
CAS No. | 1314241-44-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 9 mg/mL (26.6 mM)
Ethanol: 63 mg/mL (186.3 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 2.9565 mL | 14.7824 mL | 29.5648 mL | 73.912 mL |
5 mM | 0.5913 mL | 2.9565 mL | 5.913 mL | 14.7824 mL | |
10 mM | 0.2956 mL | 1.4782 mL | 2.9565 mL | 7.3912 mL | |
20 mM | 0.1478 mL | 0.7391 mL | 1.4782 mL | 3.6956 mL | |
Ethanol | 50 mM | 0.0591 mL | 0.2956 mL | 0.5913 mL | 1.4782 mL |
100 mM | 0.0296 mL | 0.1478 mL | 0.2956 mL | 0.7391 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
UNC 669 1314241-44-5 Chromatin/Epigenetic Epigenetic Reader Domain UNC669 UNC-669 Inhibitor L3MBTL1 L3MBTL3 repression MBT repeats inhibit transcriptional inhibitor