Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CeMMEC1 是一种 BRD4 抑制剂,对 TAF1 有很好的亲和力,IC50为0.9 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 218 | 现货 | ||
5 mg | ¥ 493 | 现货 | ||
10 mg | ¥ 828 | 现货 | ||
25 mg | ¥ 1,830 | 现货 | ||
50 mg | ¥ 3,230 | 现货 | ||
100 mg | ¥ 4,780 | 现货 | ||
500 mg | ¥ 9,870 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 547 | 现货 |
产品描述 | CeMMEC1 is an inhibitor of BRD4, and also has a great affinity for TAF1(IC50=0.9 μM). |
靶点活性 | TAF1:0.9 μM |
体外活性 | CeMMEC1 exhibits high affinity activity for the bromodomains of CREBBP, EP300, BRD9 and the second bromodomain of TAF1. |
激酶实验 | TAF1 binding assays are conducted using the EPIgeneous Binding Domain kit B. Binding is determined by the displacement of an acetylated biotin peptide from a GST-tagged TAF1 protein using HTRF with a Eu3+-conjugated GST antibody donor and streptavidin-conjugated acceptor. Compounds (CeMMEC1) are dispensed into assay plates, ProxiPlate-384 Plus using an Echo 525 Liquid Handler. Binding assays are conducted in a final volume of 20 μL with 5 nM TAF1-GST, 50 nM peptide (SGRGK (ac)GGK (ac)GLGK (ac)GGAK (ac)RHRK (biotin)-acid), 6.25 nM Streptavidin-XL665, 1:200 Anti-GST-Eu3+ cryptate and 0.1% DMSO. Assay reagents are dispensed into plates using a Multidrop combi and incubated at room temperature for 3 h. Fluorescence is measured using a PHERAstar microplate reader using the HTRF module with dual emission protocol (A = excitation of 320 nm, emmission of 665 nm, and B = excitation of 320 nm, emission of 620 nm). Raw data are processed to give an HTRF ratio (channel A/B × 10,000), which is used to generate IC50 curves |
细胞实验 | REDS3 cells were treated with CeMMEC1(10 μM) incubating for 24 hours. |
别名 | CeMMEC1 HCl |
分子量 | 336.34 |
分子式 | C19H16N2O4 |
CAS No. | 440662-09-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 21 mg/mL(62.4 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.9732 mL | 14.8659 mL | 29.7318 mL | 74.3295 mL |
5 mM | 0.5946 mL | 2.9732 mL | 5.9464 mL | 14.8659 mL | |
10 mM | 0.2973 mL | 1.4866 mL | 2.9732 mL | 7.433 mL | |
20 mM | 0.1487 mL | 0.7433 mL | 1.4866 mL | 3.7165 mL | |
50 mM | 0.0595 mL | 0.2973 mL | 0.5946 mL | 1.4866 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
CeMMEC1 440662-09-9 Cell Cycle/Checkpoint Chromatin/Epigenetic DNA Damage/DNA Repair Proteases/Proteasome Epigenetic Reader Domain Carboxypeptidase DNA/RNA Synthesis CeMMEC-1 Inhibitor CeMMEC1 HCl inhibit CeMMEC 1 inhibitor