Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SSE15206 是一种克服多药耐药性的微管聚合抑制剂,其在 HCT116 细胞中的 GI50值为 197 nM。由于癌细胞中纺锤体形成不完整,导致 G2/M 停滞,有丝分裂异常。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 153 | 现货 | ||
2 mg | ¥ 208 | 现货 | ||
5 mg | ¥ 328 | 现货 | ||
10 mg | ¥ 496 | 现货 | ||
25 mg | ¥ 892 | 现货 | ||
50 mg | ¥ 1,490 | 现货 | ||
100 mg | ¥ 2,320 | 现货 |
产品描述 | SSE15206 is a microtubule polymerization inhibitor that overcomes multidrug resistance. Causes aberrant mitosis resulting in G2/M arrest due to incomplete spindle formation in cancer cells. |
靶点活性 | Microtubule:197 nM (GI50) |
体外活性 | Treatment of cells with SSE15206 causes aberrant mitosis resulting in G2/M arrest due to incomplete spindle formation, a phenotype often associated with drugs that interfere with microtubule dynamics. SSE15206 is able to overcome resistance to chemotherapeutic drugs in different cancer cell lines including multidrug-resistant KB-V1 and A2780-Pac-Res cell lines overexpressing MDR-1, making it a promising hit for the lead optimization studies to target multidrug resistance. |
细胞实验 | Fluorescence intensity of tubulin (4?μM) in the presence of colchicine (20?μM) and SSE15206 at indicated concentrations was measured. DMSO was used as a solvent control while 50?μM nocodazole was used as a positive control for colchicine displacement. Samples were incubated for 60?min at 37℃before measurement of fluorescence (excitation at 355?nm and emission at 460?nm). KB-V1 and A2780-Pac-Res cells were incubated with 5?μM rhodamine, for 1?hour at 37℃in the presence or absence of inhibitors. Cells were then washed twice with PBS and incubated in the efflux medium (their respective growth media) in the presence of DMSO, 20?μM verapamil, and 10?μM SSE15206 for 3?hours. The percentage of rhodamine 123 positive cells was determined . Experiments were done in duplicates with 4–5 readings for each sample in each experiment. |
分子量 | 371.45 |
分子式 | C19H21N3O3S |
CAS No. | 1370046-40-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 150 mg/mL (403.82 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.6922 mL | 13.4608 mL | 26.9215 mL | 67.3038 mL |
5 mM | 0.5384 mL | 2.6922 mL | 5.3843 mL | 13.4608 mL | |
10 mM | 0.2692 mL | 1.3461 mL | 2.6922 mL | 6.7304 mL | |
20 mM | 0.1346 mL | 0.673 mL | 1.3461 mL | 3.3652 mL | |
50 mM | 0.0538 mL | 0.2692 mL | 0.5384 mL | 1.3461 mL | |
100 mM | 0.0269 mL | 0.1346 mL | 0.2692 mL | 0.673 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
SSE15206 1370046-40-4 Apoptosis Cytoskeletal Signaling Microtubule Associated Inhibitor SSE 15206 Microtubule/Tubulin inhibit SSE-15206 inhibitor