Powder: -20°C for 3 years | In solvent: -80°C for 1 year
RG13022 (Tyrphostin RG13022) 是一种酪氨酸激酶抑制剂,抑制EGF 受体自身磷酸化的IC50值为4 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 328 | 现货 | ||
5 mg | ¥ 463 | 现货 | ||
10 mg | ¥ 663 | 现货 | ||
25 mg | ¥ 1,560 | 现货 | ||
50 mg | ¥ 2,420 | 现货 | ||
100 mg | ¥ 3,590 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 485 | 现货 |
产品描述 | RG13022 (Tyrphostin RG13022) is a tyrosine kinase inhibitor; inhibits the autophosphorylation reaction of the EGF receptor (IC50: 4 μM). |
靶点活性 | EGF:4 μM |
体外活性 | RG13022 suppresses EGF-stimulated cancer cell proliferation. In a cell-free reaction, RG13022 inhibits the autophosphorylation reaction of the EGF receptor in immunoprecipitates (IC50: 4 μM). RG13022 inhibits colony formation (IC50: 1 μM) and DNA synthesis (IC50: 3 μM) by HER 14 cells (stimulated by 50 ng/mL EGF), in a dose-dependent manner. RG-13022 inhibits not only EGF-induced growth but also growth stimulated by insulin, insulin-like growth factor I, insulin-like growth factor II, or transforming growth factor alpha. RG-13022 also totally blocks estrogen-stimulated phosphorylation of the EGF receptor, as well as estrogen-induced cell proliferation, suggesting that functioning TK pathways are required for estrogen action. |
体内活性 | RG13022 suppresses tumor growth in nude mice. It also increases the lifespan of these tumor-bearing nude mice. |
细胞实验 | 40 mM stock solutions of RG13022 is made in 100% DMSO and diluted with the culture medium before addition to the cells. MH-85 cells and HER 14 cells are plated in culture medium in the presence or absence of increasing concentrations of RG-13022 or RG-14620 for 10 days. At the end of culture, the cells are fixed with 4% (v/v) formaldehyde and stained with hematoxylin. Numbers of colonies including more than 20 cells in each well are counted under the microscope[1]. |
别名 | RG 13022, Tyrphostin RG13022 |
分子量 | 266.29 |
分子式 | C16H14N2O2 |
CAS No. | 136831-48-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (187.76 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.7553 mL | 18.7765 mL | 37.553 mL | 93.8826 mL |
5 mM | 0.7511 mL | 3.7553 mL | 7.5106 mL | 18.7765 mL | |
10 mM | 0.3755 mL | 1.8777 mL | 3.7553 mL | 9.3883 mL | |
20 mM | 0.1878 mL | 0.9388 mL | 1.8777 mL | 4.6941 mL | |
50 mM | 0.0751 mL | 0.3755 mL | 0.7511 mL | 1.8777 mL | |
100 mM | 0.0376 mL | 0.1878 mL | 0.3755 mL | 0.9388 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
RG13022 136831-48-6 Angiogenesis JAK/STAT signaling Tyrosine Kinase/Adaptors EGFR HER1 Inhibitor RG 13022 Tyrphostin RG-13022 inhibit ErbB-1 Tyrphostin RG13022 Tyrphostin RG 13022 RG-13022 Epidermal growth factor receptor inhibitor