Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DIM-C-pPhCO2Me 是一种核受体 4A1 拮抗剂,具有抗肿瘤作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 247 | 现货 | ||
5 mg | ¥ 556 | 现货 | ||
10 mg | ¥ 888 | 现货 | ||
25 mg | ¥ 1,800 | 现货 | ||
50 mg | ¥ 2,790 | 现货 | ||
100 mg | ¥ 3,970 | 现货 | ||
200 mg | ¥ 5,830 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 556 | 现货 |
产品描述 | DIM-C-pPhCO2Me is a nuclear receptor 4A1 (NR4A1) antagonist. |
体外活性 | DIM-C-pPhCO2Me inhibited growth and induced apoptosis in ACHN and 786-O cells [1]. DIM-C-pPhCO2Me decreased NR4A1-dependent transactivation in RMS cells and inhibited RMS cell and tumor growth and induced apoptosis. Both NR4A1 knockdown and treatment with DIM-C-pPhCO2Me also induced ROS which activated stress genes and induced sestrin 2 which activated AMPK and inhibited mTOR in the mutant p53 RMS cells [2]. |
细胞实验 | Knockdown of NR4A1 in ACHN and 786-O cells was carried out using Lipofectamine 2000 reagent according to the manufacturer's protocol. siRNA complexes used in the study are as follows: siGL2-5', CGU ACG CGG AAU ACU UCG A; siNR4A1 (1)-SASI_Hs02_00333289; siNR4A1 (2)-SASI_Hs01_00182072. ACHN RCC cells were plated on 12-well plates at 5 x 104 cells per well in DMEM supplemented with 2.5% charcoal-stripped FBS. After 24 hr, various amounts of DNA [NBRE3-luc (400 ng), FLAG-NR4A1 (40 ng)] were cotransfected into each well by Lipofectamine 2000 reagent according to the manufacturer's protocol. After 6 hr of transfection, cells were treated with 2.5% stripped DMEM containing either DMSO, DIM-C-pPhOH (20 μM) or DIM-C-pPhCO2Me (15 μM) for 18 hr [1]. |
分子量 | 380.44 |
分子式 | C25H20N2O2 |
CAS No. | 151358-48-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (131.43 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.6285 mL | 13.1427 mL | 26.2854 mL | 65.7134 mL |
5 mM | 0.5257 mL | 2.6285 mL | 5.2571 mL | 13.1427 mL | |
10 mM | 0.2629 mL | 1.3143 mL | 2.6285 mL | 6.5713 mL | |
20 mM | 0.1314 mL | 0.6571 mL | 1.3143 mL | 3.2857 mL | |
50 mM | 0.0526 mL | 0.2629 mL | 0.5257 mL | 1.3143 mL | |
100 mM | 0.0263 mL | 0.1314 mL | 0.2629 mL | 0.6571 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
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