keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Brilliant Black BN (E 151) 是一种偶氮染料和一种食品着色剂。它抑制 EV71 与其细胞脱壳因子亲环蛋白 A 之间的相互作用,是一种很有前途的抗 EV71 感染的抗病毒药物。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 142 | 现货 | ||
2 mg | ¥ 189 | 现货 | ||
5 mg | ¥ 287 | 现货 | ||
10 mg | ¥ 397 | 现货 | ||
25 mg | ¥ 652 | 现货 | ||
50 mg | ¥ 981 | 现货 | ||
100 mg | ¥ 1,460 | 现货 | ||
500 mg | ¥ 3,660 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 388 | 现货 |
产品描述 | Brilliant Black BN (E 151) is an azo dye and a food colorant. It is a promising antiviral agent against EV71 infection via inhibiting the interaction between EV71 and its cellular uncoating factor cyclophilin A. |
靶点活性 | EV71 (various strains):2.39 µM- 28.12 µM (IC50) |
体外活性 | Brilliant black BN inhibited the infection EV71 -GFP at a concentration of 300 μM. Brilliant black BN exhibits the inhibition of EV71 -GFP as a dose-dependent manner in infected RD cells (IC50: 10.1 μM). Brilliant black BN is able to inhibit all tested 28 EV71, CVA16, and CVA6 strains. In rhabdomyosarcoma cells, 50% inhibitory concentration 29 of the dye E151 for various strains of EV71 ranged from 2.39 μM to 28.12 μM, whereas its 50% cytotoxic concentration is 1870 μM [2]. |
体内活性 | Brilliant black BN (i.p.; 200 mg/kg; 14 days)exhibits mild illness with clinical scores less than 3 and subsequently recovered when compared with PBS-group. All E151 270 treated mice are completely protected throughout the experiment [2]. |
别名 | 亮黑1, E 151 |
分子量 | 867.68 |
分子式 | C28H17N5Na4O14S4 |
CAS No. | 2519-30-4 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 123 mg/mL (141.75 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.1525 mL | 5.7625 mL | 11.525 mL | 28.8125 mL |
5 mM | 0.2305 mL | 1.1525 mL | 2.305 mL | 5.7625 mL | |
10 mM | 0.1152 mL | 0.5762 mL | 1.1525 mL | 2.8812 mL | |
20 mM | 0.0576 mL | 0.2881 mL | 0.5762 mL | 1.4406 mL | |
50 mM | 0.023 mL | 0.1152 mL | 0.2305 mL | 0.5762 mL | |
100 mM | 0.0115 mL | 0.0576 mL | 0.1152 mL | 0.2881 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Brilliant Black BN 2519-30-4 Microbiology/Virology Others Virus Protease HFMD HEVs 亮黑1 additive,contagious HEV EV71,infection HRVs antiviral,food HRV Rhinovirus Enterovirus E-151 E 151 inhibit E151 Inhibitor disease inhibitor