Powder: -20°C for 3 years | In solvent: -80°C for 1 year
BQCA (benzylquinolone carboxylic acid) 是 M1 mAChR 的一种高选择性变构调节剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 258 | 现货 | ||
5 mg | ¥ 396 | 现货 | ||
10 mg | ¥ 567 | 现货 | ||
25 mg | ¥ 1,160 | 现货 | ||
50 mg | ¥ 1,830 | 现货 | ||
100 mg | ¥ 2,890 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 455 | 现货 |
产品描述 | BQCA (benzylquinolone carboxylic acid) a highly selective allosteric M1 mAChR modulator. |
体外活性 | BQCA increases M1 receptor affinity for acetylcholine. The activation of the M1 receptor by BQCA induces a robust inward current and increases spontaneous excitatory postsynaptic currents in medial prefrontal cortex (mPFC) pyramidal cells. |
体内活性 | BQCA induces β-arrestin recruitment to M1, suggesting a role for this signal transduction mechanism in the cholinergic modulation of memory. BQCA reverses scopolamine-induced memory deficits in contextual fear conditioning, increases blood flow to the cerebral cortex, and increases wakefulness while reducing delta sleep. BQCA increases firing of mPFC pyramidal cells in vivo. BQCA also restores discrimination reversal learning in a transgenic mouse model of Alzheimer's disease. |
激酶实验 | Competition binding reactions used 25 μg human M1 CHO membrane protein, BQCA or vehicle, and 0.15 nM [3H]NMS in 96-well deep-well plates. Binding reactions (30 °C for 2-3 h) are terminated by rapid filtration. Nonspecific binding is determined by adding 10 μM atropine. Filter plates are ished 4×with ice-cold 20 mM HEPES, 100 mM NaCl, and 5 mM MgCl2, pH 7.4 using a 96-well harvester. Plates are dried and radioactivity counted with a microplate scintillation counter[1]. |
别名 | benzylquinolone carboxylic acid |
分子量 | 309.32 |
分子式 | C18H15NO4 |
CAS No. | 338747-41-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 6.25 mg/mL (20.21 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.2329 mL | 16.1645 mL | 32.329 mL | 80.8224 mL |
5 mM | 0.6466 mL | 3.2329 mL | 6.4658 mL | 16.1645 mL | |
10 mM | 0.3233 mL | 1.6164 mL | 3.2329 mL | 8.0822 mL | |
20 mM | 0.1616 mL | 0.8082 mL | 1.6164 mL | 4.0411 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
BQCA 338747-41-4 Neuroscience AChR mAChR Muscarinic acetylcholine receptor Inhibitor inhibit benzylquinolone carboxylic acid inhibitor