IL-27 Protein, Mouse, Recombinant (His)

IL-27 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 49.5 kDa and the accession number is O35228&Q8K3I6.

规格	价格/CNY		数量
20 μg	￥ 1,890	现货
100 μg	￥ 3,930	5日内发货
200 μg	￥ 6,890	5日内发货
500 μg	￥ 14,400	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 1,890

现货

100 μg

￥ 3,930

5日内发货

200 μg

￥ 6,890

5日内发货

500 μg

￥ 14,400

5日内发货

生物活性	1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc , The EC50 of human IL27RA-Fc is 0.26-0.62 μg/ml. 2. Measured in antiviral assay using HepG2 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 2-10ng/mL.
产品描述	IL-27 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 49.5 kDa and the accession number is O35228&Q8K3I6.

种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	O35228&Q8K3I6
别名	p28, Il30, IL-27, 白介素, interleukin 27, IL-27p28, 白细胞介素
蛋白构建	The Mouse IL27 complex composed of EBI3 (O35228) (Met1-Pro228) and IL27A (Q8K3I6) (Phe29-Ser234) linked by an polypeptide linker was fused with the polyhistidine tag at the C-terminus.
蛋白纯度	> 85 % as determined by SDS-PAGE
分子量	49.5 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	IL-27 protein is a member of the IL-6 superfamily, which is expressed on monocytes, endothelial cells, and dendritic cells. IL-27 protein is also referred to as the IL-12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 (EBV-induced gene 3), and IL-12 p40-related protein (IL-27B). IL-27 protein is an early product of activated antigen-presenting cells and drives the rapid clonal expansion of naive CD4(+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 protein has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy.