Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NB-598 是一种鲨烯环氧化酶竞争性抑制剂,可以阻碍甘油三酯的生物合成。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 747 | 现货 | ||
2 mg | ¥ 1,120 | 现货 | ||
5 mg | ¥ 1,890 | 现货 | ||
10 mg | ¥ 2,950 | 现货 | ||
25 mg | ¥ 4,850 | 现货 | ||
50 mg | ¥ 6,730 | 现货 | ||
100 mg | ¥ 8,900 | 现货 | ||
500 mg | ¥ 17,900 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 2,120 | 现货 |
产品描述 | NB-598 is an effective and competitive inhibitor of squalene epoxidase. It suppresses triglyceride biosynthesis through the farnesol pathway. |
体外活性 | NB-598(10 μM)能够抑制来自[14C]乙酸的固醇和固醇酯的合成,而不影响其他脂质如磷脂(PL)、游离脂肪酸(FFA)和三酰甘油(TG)的合成。在无外源性脂质体胆固醇的条件下,NB-598降低ACAT活性31%。即使在含600 pM脂质体胆固醇的环境中,NB-598也能降低ACAT活性22%[1]。NB598(10 μM)导致MIN6细胞总胆固醇水平降低36±7%。NB598显著降低PM、ER和SG处的胆固醇,分别下降49±2%、46±7%和48±2%。NB598剂量依赖性地抑制胰岛素分泌,在基础(1 mM葡萄糖)和葡萄糖刺激(16.7 mM葡萄糖)条件下均是如此。NB598在高达10 μM的浓度下,不影响KV电流的峰值外向电流或激活的电压依赖性,但会增加电流的失活[2]。 |
激酶实验 | Caco-2 cells are grown in a 58 cm2 plastic dish with medium A for 13 days. The cells are washed with medium B, and then cultured with medium B including cholesterol-micelle and each compound. The compound is dissolved in Me2SO, and the final concentration of Me2SO is 0.1%(v/v). After 18 hr of incubation, the cells are washed extensively with phosphate-buffered saline (PBS) to remove the compound. Microsomes are prepared as described above. The reaction mixture (0.2 mL) consisted of 0.1 mg microsomes, 0.25% BSA and 40 PM [14C]oleoyl CoA in buffer A. To avoid the effects of endogenous cholesterol, liposome (2 mol of cholesterol: 1 mol of phosphatidylcholine) [15] is added to the reaction mixture. The microsomes are preincubated for 1 hr with or without exogenous cholesterol, and ACAT activity is determined as described above [1]. |
分子量 | 449.67 |
分子式 | C27H31NOS2 |
CAS No. | 131060-14-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 10 mg/mL (22.24 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.2239 mL | 11.1193 mL | 22.2385 mL | 55.5963 mL |
5 mM | 0.4448 mL | 2.2239 mL | 4.4477 mL | 11.1193 mL | |
10 mM | 0.2224 mL | 1.1119 mL | 2.2239 mL | 5.5596 mL | |
20 mM | 0.1112 mL | 0.556 mL | 1.1119 mL | 2.7798 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NB-598 131060-14-5 Microbiology/Virology Antifungal inhibit Inhibitor NB598 NB 598 inhibitor