Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Chetomin (BRN0077366) 是球毛壳菌的活性成分,是一种无毒的非小细胞肺癌干细胞靶向分子。它是一种热休克蛋白90/缺氧诱导因子1α 途径的抑制剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 2,990 | 待询 | ||
5 mg | ¥ 7,970 | 待询 | ||
10 mg | ¥ 11,900 | 待询 | ||
25 mg | ¥ 21,100 | 待询 | ||
50 mg | ¥ 32,500 | 待询 | ||
100 mg | ¥ 51,070 | 待询 | ||
200 mg | ¥ 76,605 | 待询 |
产品描述 | Chetomin (BRN0077366) is an inhibitor of HIF-1 by weaken transcription of HIF-1, disrupting the binding of HIF-1α and HIF-2α to p300 at low nanomolar concentrations. |
体外活性 | HIF-1 (hypoxia-inducible factors 1) is transcription factor which respond to changes of cellular environment oxygen[2]. Chetomin selectively inhibits HIF-1 activities through disruption of the interaction of HIF-1 with its transcriptional coactivator p300. HIF-1 inhibition by chetomin effectively reduces hypoxia-dependent transcription and radiosensitizes hypoxic HT 1080 human fibrosarcoma cells in vitro[1]. Chetomin attenuates the hypoxia-induced radioresistance of malignant glioma cell lines U251 mg (DMF10: 1.35 and 1.18) and U343 mg (DMF10: 1.78 and 1.48)[3]. Targeting of HIF-1 using chetomin abolishes the differentiation-inhibitory effect of hypoxia-inducible factor-1α[4]. |
体内活性 | Administration of chetomin in combination with forskolin significantly suppresses malignant glioma growth in an in vivo xenograft model [4]. Che-M(chetomin loaded micelles) dramatically inhibits embryonic angiogenesis, tumor-induced angiogenesis and tumor growth in zebrafish. In mouse model, Che-M suppresses tumor growth and prolongs the survival in the subcutaneous CT26 tumor model [5]. |
细胞实验 | In RT-PCR and clonogenic survival experiments, chetomin is added in a concentration of 150 nM to fully supplemented medium four hours before treatment with hypoxia. HT 1080 cells are then transferred to the hypoxic workstation (0.1% O2, 12 h) or to the well-humidified incubator (12 hours) without changing medium. HT1080 cells are thus treated for 16 hours with chetomin (150 nM) prior to radiation treatment. (Only for Reference) |
别名 | Chaetomin, NSC289491, BRN0077366 |
分子量 | 710.87 |
分子式 | C31H30N6O6S4 |
CAS No. | 1403-36-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 10 mg/mL (14.06 mM)
H2O: <1 mg/mL
DMSO: 93 mg/mL (130.8 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 1.4067 mL | 7.0336 mL | 14.0673 mL | 35.1682 mL |
5 mM | 0.2813 mL | 1.4067 mL | 2.8135 mL | 7.0336 mL | |
10 mM | 0.1407 mL | 0.7034 mL | 1.4067 mL | 3.5168 mL | |
DMSO | 20 mM | 0.0703 mL | 0.3517 mL | 0.7034 mL | 1.7584 mL |
50 mM | 0.0281 mL | 0.1407 mL | 0.2813 mL | 0.7034 mL | |
100 mM | 0.0141 mL | 0.0703 mL | 0.1407 mL | 0.3517 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Chetomin 1403-36-7 Angiogenesis Apoptosis Chromatin/Epigenetic Cytoskeletal Signaling Metabolism HSP HIF Heat shock proteins targeting nontoxic Chaetomium NSCLC NSC-289491 Chaetomin hypoxia-inducible potent BRN 0077366 non-small BRN-0077366 inhibit globosum NSC 289491 Inhibitor NSC289491 BRN0077366 inhibitor